Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

This data was developed using ab76318 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling Cytokeratin 10 with ab76318 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32 mins. ab76318 Anti-Cytokeratin 10 antibody [EP1607IHCY] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal skin tissue labelling Cytokeratin 10 with unpurified ab76318.

Green - CK10, red - PI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

AbReview32704****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/6000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

This image is courtesy of an Abreview submitted by Carl Hobbs.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

This data was developed using ab76318 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human skin labelling Cytokeratin 10 with ab76318 at a concentration of 0.08µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32 mins. ab76318 Anti-Cytokeratin 10 antibody [EP1607IHCY] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunocytochemistry/Immunofluorescence analysis of HACAT cells labelling Cytokeratin 10 with purified ab76318 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

• IHC-P

AbReview32716****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

This image is courtesy of an Abreview submitted by Carl Hobbs.

• IHC-P

AbReview33016****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free (AB220806)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76318).

This image is courtesy of an Abreview submitted by Carl Hobbs.