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Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker is a rabbit recombinant monoclonal antibody that is used to detect Cytokeratin 10 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in over 125 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1607IHCY has been tried and trusted by researchers since 2009
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318), expandable thumbnail
  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/2500 - 1/5000
Notes

For unpurified use at 1/100 - 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

For unpurified use at 1/50000 - 1/100000.

Species
Rat
Dilution info
1/10000
Notes

For unpurified use at 1/50000 - 1/100000.

Species
Human
Dilution info
1/10000
Notes

For unpurified use at 1/50000 - 1/100000.

Tested
Tested

Species
Human
Dilution info
1/150
Notes

For unpurified use at 1/100 - 1/250.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

2 products for Alternative Version

Target data

Function

Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity). (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

Alternative names

Recommended products

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker is a rabbit recombinant monoclonal antibody that is used to detect Cytokeratin 10 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in over 125 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1607IHCY has been tried and trusted by researchers since 2009
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1607IHCY
Purification technique
Affinity purification Protein A
Specificity

Some customers have successfully used this antibody to staining Cytokeratin 10 in mouse tissue. Please see Abreviews for more details.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) has been cited over 129 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) has 13 independent reviews from customers.

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) specifically detects Cytokeratin 10 (UniProt ID: P13645; Molecular weight: 59kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP1607IHCY - ab2286.

Antibody clone EP1607IHCY is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, HRP (Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] ab194231, HRP Anti-Cytokeratin 10 antibody [EP1607IHCY] ab194232).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 10 also known as CK10 K10 or KRT10 is a type I keratin protein with a mass of about 59 kDa. Researchers recognize it as an important structural protein found mainly in the suprabasal layers of the epidermis where it contributes to maintaining the integrity and function of the skin barrier. Cytokeratin 10 is also expressed in other stratified squamous epithelia. Scientists use antibodies against cytokeratin 10 sometimes called 'lh2' and 'cytokeratin 10 anticuerpos' as neomarkers for identifying specific cell types in tissue studies.

Biological function summary

Keratin proteins like cytokeratin 10 provide mechanical stability to cells and tissues. Cytokeratin 10 forms a heterodimer with keratin 1 (KRT1) creating a complex that plays a critical role in the cytoskeleton of keratinocytes. This structure supports cellular resilience against mechanical stresses helping maintain the integrity of epithelial tissues. Keratin 10 function is essential for normal skin development and differentiation highlighting its significance in epidermal morphogenesis.

Pathways

Cytokeratin 10 is integral to the epidermal differentiation complex. This pathway involves many regulatory proteins and is essential for proper skin formation and repair. Cytokeratin 10 together with KRT1 contributes to the terminal differentiation of keratinocytes. Another key pathway is the cornification process where cytokeratin 10 helps form the protective outer layer of the skin. These biological roles place cytokeratin 10 at an essential point in maintaining skin homeostasis and function.

Associated diseases and disorders

Abnormal expression or mutation of cytokeratin 10 leads to skin conditions like epidermolytic hyperkeratosis. This rare inherited disorder results in blistering and thickening of the skin. Cytokeratin 10 mutations often appear alongside changes in KRT1 due to their forming a functional pair indicating their collaborative role in disease manifestation. Additionally altered cytokeratin 10 expression can be associated with psoriasis as disrupted keratinocyte differentiation contributes to this inflammatory skin disease. Understanding these associations helps in studying and developing treatment strategies for skin disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Immunocytochemistry/Immunofluorescence analysis of HACAT cells labelling Cytokeratin 10 with purified ab76318 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/500) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/10000 dilution

    Lane 1: HACAT cell lysate at 20 µg

    Lane 2: A431 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution

    All lanes: Human fetal skin tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution

    All lanes: Rat skin tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal skin tissue labelling Cytokeratin 10 with unpurified ab76318.

    Green - CK10, red - PI.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/200000 dilution

    All lanes: Mouse skin tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318), expandable thumbnail

    Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

    All lanes: Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/100000 dilution

    All lanes: HaCat cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 59 kDa

    Observed band size: 60 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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