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Rabbit Monoclonal Cytokeratin 13 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 44 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100000 - 1/500000

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Alternative names

Recommended products

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Rabbit Monoclonal Cytokeratin 13 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 44 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3671

Purification technique

Affinity purification Protein A

Dissociation constant

1.2 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

Biological function summary

This protein participates in maintaining the structural framework and dynamics within epithelial tissues. As part of the cytoskeletal complex cytokeratin 13 supports cellular resilience against mechanical stress. It interacts with other cytokeratins contributing to the formation of a robust keratin filament network essential for epithelial cell function and differentiation. Its expression and regulation are key to the homeostasis of the tissues where it is found ensuring proper cell division and tissue integrity.

Activity summary

Cytokeratin 13 also known as KRT13 or CK13 is a type I intermediate filament protein weighing approximately 44 kDa. It is one of the cytokeratin types that plays a critical role in the mechanical stability and integrity of epithelial cells. Cytokeratin 13 is expressed in the stratified squamous epithelium particularly in the upper layers of non-cornified epithelia which include the esophagus tongue and cervix. This protein forms heteropolymers with type II keratins contributing to the cytoskeletal filament network within epithelial cells.

Pathways

Cytokeratin 13 integrates into epithelial cell differentiation and repair processes. It is involved in the epithelial-mesenchymal transition (EMT) pathway important for tissue development wound healing and cancer metastasis. During the EMT process cytokeratin 13 often works alongside proteins like E-cadherin and Vimentin mediating changes in epithelial cell architecture and polarity. This modulation is essential for cellular plasticity and response to environmental cues.

Associated diseases and disorders

Cytokeratin 13 has associations with certain epithelial tissue abnormalities such as oral squamous cell carcinoma and a subgroup of inherited blistering diseases like epidermolysis bullosa. In oral squamous cell carcinoma dysregulation of cytokeratin 13 expression may indicate tumor progression and metastasis. In epidermolysis bullosa mutations affecting keratin interactions including those with other keratins like cytokeratin 14 can lead to fragile skin and mucosal surfaces resulting in increased susceptibility to physical damage and impaired healing.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    False colour image of Western blot: Anti-Cytokeratin 13 antibody [EPR3671] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92551 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line ab269483 (knockout cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551) at 1/100000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: KRT13 knockout A431 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 50 kDa

    Observed band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    All lanes: Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551) at 1/100000 dilution

    Lane 1: A431 cell lysate at 10 µg

    Lane 2: HACAT cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Secondary

    Lanes 1 - 2: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Lane 3: HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

    Confocal image showing cytoplasmic staining in A431 cells.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing positive staining in Cervical human carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail

    OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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