Anti-Cytokeratin 13 antibody [EPR3671]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

ab92551 showing positive staining in Cervical human carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

ab92551 staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell urinary bladder carcinoma tissue by immunohistochemistry. Detection : DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

Confocal image showing cytoplasmic staining in A431 cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

All lanes:

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HACAT cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

Lanes 1 - 2:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Lane 3:

HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 50 kDa

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• WB

Lab

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

False colour image of Western blot : Anti-Cytokeratin 13 antibody [EPR3671] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92551 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line ab269483 (knockout cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

KRT13 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human KRT13 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-krt13-knockout-a-431-cell-line-ab269483'>ab269483</a>)

Predicted band size: 50 kDa

Observed band size: 51 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.