Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) is a rabbit monoclonal antibody detecting Cytokeratin 13 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
- Trusted since 2010
Images
![Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--western-blot-img170346.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37904.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)")
![Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--western-blot-img106258.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunocytochemistry-immunofluorescence-img18697.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)")
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--flow-cytometry-intracellular-img26821.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (AB92551)")
Publications
Filter by
- Cells 12:2023Opposing Roles of Blood-Borne Monocytes and Tissue-Resident Macrophages in Limbal Stem Cell Damage after Ocular Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengxin Zhou,Fengyang Lei,Mirja Mittermaier,Bruce Ksander,Reza Dana,Claes H Dohlman,Demetrios G Vavvas,James Chodosh,Eleftherios I PaschalisPubMed 37626899
- Scientific reports 13:122612023Full thickness 3D in vitro conjunctiva model enables goblet cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Schwebler,Christina Fey,Daniel Kampik,Christian LotzPubMed 37507439
- International journal of molecular sciences 24:2023Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Toshida,Tomoto Kasahara,Masamichi Kiriyama,Yuma Iwasaki,Jobu Sugita,Kohei Ichikawa,Toshihiko Ohta,Katsumi MiyaharaPubMed 37240280
- Nature protocols 18:2283-23122023A distal lung organoid model to study interstitial lung disease, viral infection and human lung development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Matkovic Leko,Remy T Schneider,Tania A Thimraj,Nadine Schrode,Daniel Beitler,Hsiao-Yun Liu,Kristin Beaumont,Ya-Wen Chen,Hans-Willem SnoeckPubMed 37165073
- eLife 12:2023Autofluorescence imaging permits label-free cell type assignment and reveals the dynamic formation of airway secretory cell associated antigen passages (SAPs).Applications:Unspecified applicationReactive species:Unspecified reactive speciesViral S Shah,Jue Hou,Vladimir Vinarsky,Jiajie Xu,Manalee V Surve,Charles P Lin,Jayaraj RajagopalPubMed 36994985
- Cell reports 41:1115032022Mutant Ras and inflammation-driven skin tumorigenesis is suppressed via a JNK-iASPP-AP1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhatoun Al Moussawi,Kathryn Chung,Thomas M Carroll,Christian Osterburg,Artem Smirnov,Rebecca Lotz,Paul Miller,Zinaida Dedeić,Shan Zhong,Martin Oti,Evelyn N Kouwenhoven,Ruth Asher,Robert Goldin,Michael Tellier,Shona Murphy,Huiqing Zhou,Volker Dötsch,Xin LuPubMed 36261000
- Breast cancer research : BCR 24:72022KRT13 promotes stemness and drives metastasis in breast cancer through a plakoglobin/c-Myc signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Yin,Qinlong Li,Stefan Mrdenovic,Gina Chia-Yi Chu,Boyang Jason Wu,Hong Bu,Peng Duan,Jayoung Kim,Sungyong You,Michael S Lewis,Gangning Liang,Ruoxiang Wang,Haiyen E Zhau,Leland W K ChungPubMed 35078507
- iScience 24:1034402021A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiyun Zheng,Kyung-Pil Ko,Xuefen Fang,Xiaozhong Wang,Jie Zhang,Sohee Jun,Bong-Jun Kim,Wenyi Luo,Moon Jong Kim,Youn-Sang Jung,Christopher L Cervantes,Jae-Il ParkPubMed 34877497
- Cell regeneration (London, England) 10:362021Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZixian Zhao,Yujia Wang,Yingchuan Wu,Dandan Li,Ting Zhang,Yu Ma,Xiaoming Teng,Wei ZuoPubMed 34719766
- International journal of molecular sciences 22:2021Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara I Van Acker,Bert Van den Bogerd,Zoë P Van Acker,Agnė Vailionytė,Michel Haagdorens,Carina Koppen,Sorcha Ní Dhubhghaill,Darlene A Dartt,Isabel PintelonPubMed 34638869
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100000 - 1/500000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2 (By similarity).
Alternative names
Cytokeratin-13, Keratin-13, CK-13, K13, KRT13
Recommended products
Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) is a rabbit monoclonal antibody detecting Cytokeratin 13 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3671
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.2 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Cytokeratin 13?
Anti-Cytokeratin 13 [EPR3671] (ab92551) specifically detects a band for Cytokeratin 13 (UniProt: P13646) at a molecular weight of 50kDa.
Trusted by the scientific community
Anti-Cytokeratin 13 [EPR3671] (ab92551) was first used in a scientific publication in 2010 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) has been confirmed by Western blot testing in Cytokeratin 13 Knockout A-431 cell line, Human KRT13 knockout A-431 cell line ab269483.
Other related products
We have a range of other formats of antibody clone [EPR3671] also available for your convenience:
ab92551, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Cytokeratin 13 antibody [EPR3671] ab198584, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Cytokeratin 13 antibody [EPR3671] ab198585, HRP - HRP Anti-Cytokeratin 13 antibody [EPR3671] ab199092, Carrier free - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free ab239918, APC - APC Anti-Cytokeratin 13 antibody [EPR3671] ab319321, PE - PE Anti-Cytokeratin 13 antibody [EPR3671] ab319459, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Cytokeratin 13 antibody [EPR3671] ab319813, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Cytokeratin 13 antibody [EPR3671] ab319954, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Cytokeratin 13 antibody [EPR3671] ab321765
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 13 also known as KRT13 or CK13 is a type I intermediate filament protein weighing approximately 44 kDa. It is one of the cytokeratin types that plays a critical role in the mechanical stability and integrity of epithelial cells. Cytokeratin 13 is expressed in the stratified squamous epithelium particularly in the upper layers of non-cornified epithelia which include the esophagus tongue and cervix. This protein forms heteropolymers with type II keratins contributing to the cytoskeletal filament network within epithelial cells.
- Biological function summary
This protein participates in maintaining the structural framework and dynamics within epithelial tissues. As part of the cytoskeletal complex cytokeratin 13 supports cellular resilience against mechanical stress. It interacts with other cytokeratins contributing to the formation of a robust keratin filament network essential for epithelial cell function and differentiation. Its expression and regulation are key to the homeostasis of the tissues where it is found ensuring proper cell division and tissue integrity.
- Pathways
Cytokeratin 13 integrates into epithelial cell differentiation and repair processes. It is involved in the epithelial-mesenchymal transition (EMT) pathway important for tissue development wound healing and cancer metastasis. During the EMT process cytokeratin 13 often works alongside proteins like E-cadherin and Vimentin mediating changes in epithelial cell architecture and polarity. This modulation is essential for cellular plasticity and response to environmental cues.
- Associated diseases and disorders
Cytokeratin 13 has associations with certain epithelial tissue abnormalities such as oral squamous cell carcinoma and a subgroup of inherited blistering diseases like epidermolysis bullosa. In oral squamous cell carcinoma dysregulation of cytokeratin 13 expression may indicate tumor progression and metastasis. In epidermolysis bullosa mutations affecting keratin interactions including those with other keratins like cytokeratin 14 can lead to fragile skin and mucosal surfaces resulting in increased susceptibility to physical damage and impaired healing.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--western-blot-img170346.jpg "Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
False colour image of Western blot: Anti-Cytokeratin 13 antibody [EPR3671] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92551 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line Human KRT13 knockout A-431 cell line ab269483 (knockout cell lysate Human KRT13 knockout A-431 cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: KRT13 knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human KRT13 knockout A-431 cell line (Human KRT13 knockout A-431 cell line ab269483)
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37904.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
Cytokeratin 13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Cytokeratin 13 antibody
ab92551 staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell urinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
![Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--western-blot-img106258.jpg "Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
All lanes: Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution
Lane 1: A431 cell lysate at 10 µg
Lane 2: HACAT cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
SecondaryLanes 1 - 2: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Lane 3: HRP labelled goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 50 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunocytochemistry-immunofluorescence-img18697.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Confocal image showing cytoplasmic staining in A431 cells.
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--flow-cytometry-intracellular-img26821.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59857.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
ab92551 showing positive staining in Cervical human carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113051.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133541.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129882.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--oi-rd-scanning-img95298.jpg "OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59857.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113051.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133541.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129882.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
![OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-13-antibody-epr3671-ab92551--oi-rd-scanning-img95298.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com