Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

ab92551 showing positive staining in human Cervical carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

ab92551 staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell urinary bladder carcinoma tissue by immunohistochemistry. Detection : DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

Confocal image showing cytoplasmic staining in A431 cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

• WB

Lab

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

False colour image of Western blot : Anti-Cytokeratin 13 antibody [EPR3671] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92551 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line ab269483 (knockout cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92551).

All lanes:

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (<a href='/en-us/products/primary-antibodies/cytokeratin-13-antibody-epr3671-ab92551'>ab92551</a>) at 1/100000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

KRT13 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human KRT13 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-krt13-knockout-a-431-cell-line-ab269483'>ab269483</a>)

Predicted band size: 50 kDa

Observed band size: 51 kDa

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• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918)

Cytokeratin 13 western blot using anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free ab239918. Publication image and figure legend from Tumpara, S., Martínez-Delgado, B., et al., 2020, Front Pharmacol, PubMed 32719598.

ab239918 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab239918 please see the product overview.

Effect of AAT on KRT 4 (A) and KRT 13 (B) proteins. EpiCS were cultured for 18 h with topically applied HBSS (control) or with 0.2 and 0.5 mg human AAT. Cell lysates were subjected to Western blot analysis using anti-KRT4 and anti-KRT13 antibody (1 : 500). β-actin was used as a loading control. Representative blots are shown. Bars show relative intensity of KRT4/13 bands from two independent experiments.

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