Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)

Rabbit Monoclonal Cytokeratin 13 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (AB239918), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Tested	Tested
Mouse	Predicted	Predicted	Not recommended	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Associated Products

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3 products for Alternative Version

Target data

See full target information Keratin, type I cytoskeletal 13

Function

Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2 (By similarity).

Alternative names

Cytokeratin-13, Keratin-13, CK-13, K13, KRT13

Recommended products

Rabbit Monoclonal Cytokeratin 13 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR3671
Purification technique: Affinity purification Protein A
Dissociation constant: 1.2 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab239918 is the carrier-free version of Anti-Cytokeratin 13 antibody [EPR3671] ab92551.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 13 also known as KRT13 or CK13 is a type I intermediate filament protein weighing approximately 44 kDa. It is one of the cytokeratin types that plays a critical role in the mechanical stability and integrity of epithelial cells. Cytokeratin 13 is expressed in the stratified squamous epithelium particularly in the upper layers of non-cornified epithelia which include the esophagus tongue and cervix. This protein forms heteropolymers with type II keratins contributing to the cytoskeletal filament network within epithelial cells.

Biological function summary

This protein participates in maintaining the structural framework and dynamics within epithelial tissues. As part of the cytoskeletal complex cytokeratin 13 supports cellular resilience against mechanical stress. It interacts with other cytokeratins contributing to the formation of a robust keratin filament network essential for epithelial cell function and differentiation. Its expression and regulation are key to the homeostasis of the tissues where it is found ensuring proper cell division and tissue integrity.

Pathways

Cytokeratin 13 integrates into epithelial cell differentiation and repair processes. It is involved in the epithelial-mesenchymal transition (EMT) pathway important for tissue development wound healing and cancer metastasis. During the EMT process cytokeratin 13 often works alongside proteins like E-cadherin and Vimentin mediating changes in epithelial cell architecture and polarity. This modulation is essential for cellular plasticity and response to environmental cues.

Associated diseases and disorders

Cytokeratin 13 has associations with certain epithelial tissue abnormalities such as oral squamous cell carcinoma and a subgroup of inherited blistering diseases like epidermolysis bullosa. In oral squamous cell carcinoma dysregulation of cytokeratin 13 expression may indicate tumor progression and metastasis. In epidermolysis bullosa mutations affecting keratin interactions including those with other keratins like cytokeratin 14 can lead to fragile skin and mucosal surfaces resulting in increased susceptibility to physical damage and impaired healing.

Product promise

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10 product images

Western blot - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
False colour image of Western blot: Anti-Cytokeratin 13 antibody [EPR3671] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Cytokeratin 13 antibody [EPR3671] ab92551 was shown to bind specifically to Cytokeratin 13. A band was observed at 51 kDa in wild-type A431 cell lysates with no signal observed at this size in Krt13 knockout cell line Human KRT13 knockout A-431 cell line ab269483 (knockout cell lysate Human KRT13 knockout A-431 cell lysate ab269647). To generate this image, wild-type and Krt13 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
All lanes: Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (Anti-Cytokeratin 13 antibody [EPR3671] ab92551) at 1/100000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: KRT13 knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human KRT13 knockout A-431 cell line (Human KRT13 knockout A-431 cell line ab269483)
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Cytokeratin 13 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Cytokeratin 13 antibody
Anti-Cytokeratin 13 antibody [EPR3671] ab92551 staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell urinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with Anti-Cytokeratin 13 antibody [EPR3671] ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Confocal image showing cytoplasmic staining in A431 cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Flow Cytometry (Intracellular) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified Anti-Cytokeratin 13 antibody [EPR3671] ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Anti-Cytokeratin 13 antibody [EPR3671] ab92551 showing positive staining in human Cervical carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Anti-Cytokeratin 13 antibody [EPR3671] ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Anti-Cytokeratin 13 antibody [EPR3671] ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Anti-Cytokeratin 13 antibody [EPR3671] ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 13 antibody [EPR3671] ab92551).
OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free (ab239918)
Cytokeratin 13 western blot using anti-Cytokeratin 13 antibody [EPR3671] - BSA and Azide free ab239918. Publication image and figure legend from Tumpara, S., Martínez-Delgado, B., et al., 2020, Front Pharmacol, PubMed 32719598.

ab239918 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab239918 please see the product overview.
Effect of AAT on KRT 4 (A) and KRT 13 (B) proteins. EpiCS were cultured for 18 h with topically applied HBSS (control) or with 0.2 and 0.5 mg human AAT. Cell lysates were subjected to Western blot analysis using anti-KRT4 and anti-KRT13 antibody (1:500). β-actin was used as a loading control. Representative blots are shown. Bars show relative intensity of KRT4/13 bands from two independent experiments.