AB15461

Anti-Cytokeratin 14 antibody

Name: Anti-Cytokeratin 14 antibody (ab15461) | Abcam
Brand: Abcam Limited
SKU: AB15461
Price: 290 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal Cytokeratin 14 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications.

View Alternative Names

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody (AB15461)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody (AB15461)

Human Skin stained with anti-Cytokeratin 14 antibody, ab15461 by Immunohistochemistry (FFPE-sections).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC oral health 22:165 PubMed35524231

2022

A new scoring system facilitating diagnosis of oral squamous malignancy on biopsy specimens.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Lin Wu,Cheng-Chih Huang,Shang-Yin Wu,Shih-Sheng Jiang,Fang-Yu Tsai,Jenn-Ren Hsiao

Frontiers in cell and developmental biology 8:605084 PubMed33425910

2020

Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Simancas Escorcia,Abdoulaziz Diarra,Adrien Naveau,Arnaud Dessombz,Rufino Felizardo,Vidjeacoumary Cannaya,Christos Chatziantoniou,Mickaël Quentric,Miikka Vikkula,Olivier Cases,Ariane Berdal,Muriel De La Dure-Molla,Renata Kozyraki

Frontiers in cell and developmental biology 8:461 PubMed32612992

2020

Expression of ncRNAs on the DLK1-DIO3 Locus Is Associated With Basal and Mesenchymal Phenotype in Breast Epithelial Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zuzana Budkova,Anna Karen Sigurdardottir,Eirikur Briem,Jon Thor Bergthorsson,Snævar Sigurdsson,Magnus Karl Magnusson,Gunnhildur Asta Traustadottir,Thorarinn Gudjonsson,Bylgja Hilmarsdottir

The Journal of pathology 250:362-373 PubMed31875961

2020

Loss of GATA4 causes ectopic pancreas in the stomach.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Rodríguez-Seguel,Laura Villamayor,Noelia Arroyo,Mónica P De Andrés,Francisco X Real,Franz Martín,David A Cano,Anabel Rojas

In vitro cellular & developmental biology. Animal 55:838-853 PubMed31482369

2019

YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Morera,Sarah Sophie Steinhäuser,Zuzana Budkova,Saevar Ingthorsson,Jennifer Kricker,Aileen Krueger,Gunnhildur Asta Traustadottir,Thorarinn Gudjonsson

PLoS computational biology 12:e1004924 PubMed27253373

2016

EGFR Signal-Network Reconstruction Demonstrates Metabolic Crosstalk in EMT.

Applications

Species

Unspecified reactive species

Kumari Sonal Choudhary,Neha Rohatgi,Skarphedinn Halldorsson,Eirikur Briem,Thorarinn Gudjonsson,Steinn Gudmundsson,Ottar Rolfsson

Journal of anatomy 229:302-13 PubMed27168421

2016

Characterization of the myoepithelial cells in the major salivary glands of the fruit bat Artibeus jamaicensis.

Applications

Unspecified application

Species

Unspecified reactive species

Julio Guerrero-Hernández,Norma Moreno-Mendoza

Oncogene 35:4244-55 PubMed26686087

2015

HER2 induced EMT and tumorigenicity in breast epithelial progenitor cells is inhibited by coexpression of EGFR.

Applications

IHC, WB

Species

Human, Human

S Ingthorsson,K Andersen,B Hilmarsdottir,G M Maelandsmo,M K Magnusson,T Gudjonsson

Journal of cellular biochemistry 116:903-22 PubMed25639585

2015

Characterisation of the kynurenine pathway in skin-derived fibroblasts and keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Diba Sheipouri,Ross Grant,Sonia Bustamante,David Lovejoy,Gilles J Guillemin,Nady Braidy

PloS one 8:e70408 PubMed23894651

2013

Fetal fibroblasts and keratinocytes with immunosuppressive properties for allogeneic cell-based wound therapy.

Applications

Flow Cyt

Species

Human

Thomas Zuliani,Soraya Saiagh,Anne-Chantal Knol,Julie Esbelin,Brigitte Dréno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody (AB15461)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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