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Mouse Monoclonal K1C15 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT15.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078), expandable thumbnail
  • Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078), expandable thumbnail
  • Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078), expandable thumbnail
  • Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human KRT15. Database link P19012

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBProtein ArrayIHC-P
Human
Tested
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

1 products for Alternative Version

Target data

Function

Alternative names

Cytokeratin-15, Keratin-15, CK-15, K15, KRTB, KRT15

Recommended products

Mouse Monoclonal K1C15 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT15.

Alternative names

Cytokeratin-15, Keratin-15, CK-15, K15, KRTB, KRT15

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human KRT15. Database link P19012
Clone number

KRT15/2958

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from Bioreactor concentrate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

  • Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078), expandable thumbnail

    Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078)

    Flow cytometric analysis of PFA-fixed HeLa cells labeling Cytokeratin 15 using ab268078 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

  • Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078), expandable thumbnail

    Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078)

    All lanes: Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078) at 2 µg/mL

    All lanes: Human thymus lysate

    Predicted band size: 49 kDa

  • Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078), expandable thumbnail

    Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078)

    Protein Array containing more than 19,000 full-length human proteins using ab268078. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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