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AB268078

Anti-Cytokeratin 15 antibody [KRT15/2958]

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(1 Publication)

Mouse Monoclonal K1C15 antibody. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT15.

View Alternative Names

KRTB, KRT15, Cytokeratin-15, Keratin-15, CK-15, K15

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)

Flow cytometric analysis of PFA-fixed HeLa cells labeling Cytokeratin 15 using ab268078 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)
  • WB

Unknown

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)

All lanes:

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078) at 2 µg/mL

All lanes:

Human thymus lysate

Predicted band size: 49 kDa

false

Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)
  • Protein Array

Unknown

Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] (AB268078)

Protein Array containing more than 19,000 full-length human proteins using ab268078. Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KRT15/2958

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Protein Array, WB, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT15.

P19012

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor concentrate
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 15 often abbreviated as CK15 bsa-15 or keratin 15 is an intermediate filament protein known for its role in the structural integrity of epithelial cells. It weighs approximately 50 kilodaltons. This protein is mainly expressed in the basal layer of stratified squamous epithelia such as the epidermis and the hair follicle. CK15 is an essential part of the cytoskeleton contributing to cell shape and mechanical resilience.
Biological function summary

CK15 plays a significant role in maintaining epithelial stem cell populations. It is part of the keratin protein family where it forms a complex network with other cytokeratins to support epithelial cell stability. CK15 has been shown to mark epithelial progenitor cells hinting at its role in tissue regeneration and repair. Its expression levels often reflect the cellular origin and differentiation state particularly in the skin and associated structures.

Pathways

CK15 is involved in pathways related to epithelial cell differentiation and homeostasis. It interacts within the cytoskeletal network alongside other cytokeratins like keratin 5. CK15 contributes to pathways that regulate cell proliferation and differentiation. It participates in the wound healing process where keratin 6 and keratin 17 also play important roles. This involvement allows it to impact how epithelial cells respond to and repair after damage.

CK15 has been associated with skin disorders and certain types of cancers. Its expression is often altered in squamous cell carcinoma and basal cell carcinoma where abnormal CK15 levels can influence tumor behavior. Additionally changes in CK15 expression are linked to alopecia relating it to proteins like keratin 5 and keratin 8 which have similar expression patterns in hair follicles. Understanding CK15's role may aid in developing biomarkers for diagnosis and therapeutic targets.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37895104

2023

Human Non-Small Cell Lung Cancer-Chicken Embryo Chorioallantoic Membrane Tumor Models for Experimental Cancer Treatments.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Tereza Brachtlova,Ida H van der Meulen-Muileman,Stijn Kleerebezem,Chang Liu,Peiyu Li,Victor W van Beusechem
View all publications

Product promise

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