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AB268199

Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal K1C15 antibody. Carrier free. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT15.

View Alternative Names

KRTB, KRT15, Cytokeratin-15, Keratin-15, CK-15, K15

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)

Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 15 using ab268078 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
  • WB

Unknown

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

All lanes:

Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (<a href='/en-us/products/primary-antibodies/cytokeratin-15-antibody-krt15-2958-ab268078'>ab268078</a>) at 2 µg/mL

All lanes:

Human thymus lysate

Predicted band size: 49 kDa

false

Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
  • Protein Array

Unknown

Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)

Protein Array containing more than 19,000 full-length human proteins using ab268078.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KRT15/2958

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P, Protein Array, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT15.

P19012

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

ab268199 is the carrier-free version of ab268078.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 15 often abbreviated as CK15 bsa-15 or keratin 15 is an intermediate filament protein known for its role in the structural integrity of epithelial cells. It weighs approximately 50 kilodaltons. This protein is mainly expressed in the basal layer of stratified squamous epithelia such as the epidermis and the hair follicle. CK15 is an essential part of the cytoskeleton contributing to cell shape and mechanical resilience.
Biological function summary

CK15 plays a significant role in maintaining epithelial stem cell populations. It is part of the keratin protein family where it forms a complex network with other cytokeratins to support epithelial cell stability. CK15 has been shown to mark epithelial progenitor cells hinting at its role in tissue regeneration and repair. Its expression levels often reflect the cellular origin and differentiation state particularly in the skin and associated structures.

Pathways

CK15 is involved in pathways related to epithelial cell differentiation and homeostasis. It interacts within the cytoskeletal network alongside other cytokeratins like keratin 5. CK15 contributes to pathways that regulate cell proliferation and differentiation. It participates in the wound healing process where keratin 6 and keratin 17 also play important roles. This involvement allows it to impact how epithelial cells respond to and repair after damage.

CK15 has been associated with skin disorders and certain types of cancers. Its expression is often altered in squamous cell carcinoma and basal cell carcinoma where abnormal CK15 levels can influence tumor behavior. Additionally changes in CK15 expression are linked to alopecia relating it to proteins like keratin 5 and keratin 8 which have similar expression patterns in hair follicles. Understanding CK15's role may aid in developing biomarkers for diagnosis and therapeutic targets.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 11: PubMed37072348

2023

Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua Liang
View all publications

Product promise

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