Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free
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(1 Publication)
Mouse Monoclonal K1C15 antibody. Carrier free. Suitable for Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT15.
View Alternative Names
KRTB, KRT15, Cytokeratin-15, Keratin-15, CK-15, K15
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
- Flow Cyt
Unknown
Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 15 using ab268078 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
- WB
Unknown
Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
All lanes:
Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] (<a href='/en-us/products/primary-antibodies/cytokeratin-15-antibody-krt15-2958-ab268078'>ab268078</a>) at 2 µg/mL
All lanes:
Human thymus lysate
Predicted band size: 49 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (AB268199)
Protein Array containing more than 19,000 full-length human proteins using ab268078.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
Reactivity data
Product details
ab268199 is the carrier-free version of ab268078.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CK15 plays a significant role in maintaining epithelial stem cell populations. It is part of the keratin protein family where it forms a complex network with other cytokeratins to support epithelial cell stability. CK15 has been shown to mark epithelial progenitor cells hinting at its role in tissue regeneration and repair. Its expression levels often reflect the cellular origin and differentiation state particularly in the skin and associated structures.
Pathways
CK15 is involved in pathways related to epithelial cell differentiation and homeostasis. It interacts within the cytoskeletal network alongside other cytokeratins like keratin 5. CK15 contributes to pathways that regulate cell proliferation and differentiation. It participates in the wound healing process where keratin 6 and keratin 17 also play important roles. This involvement allows it to impact how epithelial cells respond to and repair after damage.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal for immunotherapy of cancer 11: PubMed37072348
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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