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Rabbit Recombinant Monoclonal K1C16 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 21 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (AB76416), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (AB76416), expandable thumbnail
  • Western blot - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (AB76416), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (AB76416), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (AB76416), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/150
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/150
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Alternative names

KRT16A, KRT16, Cytokeratin-16, Keratin-16, CK-16, K16

Recommended products

Rabbit Recombinant Monoclonal K1C16 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 21 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1615Y
Purification technique
Affinity purification Protein A
Specificity

Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 16 often called K16 or Krt16 weighs around 50 kDa and belongs to the keratin family. Researchers commonly find K16 in epithelial tissues particularly in areas subjected to constant mechanical stress such as palms and soles. In addition to its localized expression K16 appears during wound healing processes where cells experience high turnover or regeneration activities. Its expression often pairs with Krt6 another keratin type contributing to its mechanical properties under stress conditions often called "pressure point".

Biological function summary

K16 plays critical roles in maintaining the integrity of epithelial cells and tissues. It is a structural protein that helps stabilize keratin cytoarchitecture. K16 forms a complex with its partner keratins like K17 providing resilience and elasticity to the skin ensuring durability in regions experiencing repetitive physical stress. Additionally K16 helps regulate proliferation and differentiation of keratinocytes contributing to healthy skin formation and repair.

Pathways

K16 takes part in signaling pathways associated with epidermal development and repair. It acts within the keratinization process interacting closely with signaling components that influence cell cycle regulation and DNA repair. Proteins such as p63 and Krt17 assist this regulatory mechanism influencing cellular responses to stress or damage. These interactions help modulate barrier function and skin homeostasis ensuring responsive adaptation to environmental changes.

Associated diseases and disorders

K16 links to conditions like psoriasis and pachyonychia congenita. In psoriasis K16 expression becomes markedly upregulated contributing to the characteristic skin hyperproliferation. Similarly in pachyonychia congenita mutations in the K16 gene lead to nail and skin anomalies causing painful epidermal lesions. Both disorders highlight K16's involvement with Krt6 and Krt17 as these proteins collectively demonstrate disrupted keratin networks resulting in compromised skin integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    ab76416 staining Cytokeratin 16/K16 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    ab76416 staining Cytokeratin 16/K16 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Western blot - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    All lanes: Western blot - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416) at 1/10000 dilution

    All lanes: HACAT (human keratinocyte) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/20000 dilution

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    ab76416 staining Cytokeratin 16/K16 in A431 (human epidermoid carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-EEF2/Elongation factor 2 antibody [EP880Y] ab75748 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
    Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    ab76416 staining Cytokeratin 16/K16 in HACAT (human keratinocyte) cell lineby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416)

    Overlay histogram showing HepG2 cells stained with unpurified ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS/10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76416, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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