AB181055

Anti-Cytokeratin 16/K16 antibody [EPR13504]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal K1C16 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KRT16A, KRT16, Cytokeratin-16, Keratin-16, CK-16, K16

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 16/K16 with ab181055 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Cytokeratin 16/K16 with ab181055 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

All lanes:

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (ab181055) at 1/100000 dilution

All lanes:

Human skin lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

All lanes:

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (ab181055) at 1/100000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 51 kDa

false

Carrier free

Anti-Cytokeratin 16/K16 antibody [EPR13504] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13504

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 16 often called K16 or Krt16 weighs around 50 kDa and belongs to the keratin family. Researchers commonly find K16 in epithelial tissues particularly in areas subjected to constant mechanical stress such as palms and soles. In addition to its localized expression K16 appears during wound healing processes where cells experience high turnover or regeneration activities. Its expression often pairs with Krt6 another keratin type contributing to its mechanical properties under stress conditions often called "pressure point".

Biological function summary

K16 plays critical roles in maintaining the integrity of epithelial cells and tissues. It is a structural protein that helps stabilize keratin cytoarchitecture. K16 forms a complex with its partner keratins like K17 providing resilience and elasticity to the skin ensuring durability in regions experiencing repetitive physical stress. Additionally K16 helps regulate proliferation and differentiation of keratinocytes contributing to healthy skin formation and repair.

Pathways

K16 takes part in signaling pathways associated with epidermal development and repair. It acts within the keratinization process interacting closely with signaling components that influence cell cycle regulation and DNA repair. Proteins such as p63 and Krt17 assist this regulatory mechanism influencing cellular responses to stress or damage. These interactions help modulate barrier function and skin homeostasis ensuring responsive adaptation to environmental changes.

K16 links to conditions like psoriasis and pachyonychia congenita. In psoriasis K16 expression becomes markedly upregulated contributing to the characteristic skin hyperproliferation. Similarly in pachyonychia congenita mutations in the K16 gene lead to nail and skin anomalies causing painful epidermal lesions. Both disorders highlight K16's involvement with Krt6 and Krt17 as these proteins collectively demonstrate disrupted keratin networks resulting in compromised skin integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach : required for some inflammatory checkpoint for the skin barrier maintenance.

See full target information KRT16

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gynecologic oncology 167:96-106 PubMed35918200

2022

A novel cell line panel reveals non-genetic mediators of platinum resistance and phenotypic diversity in high grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J I Hoare,H Hockings,J Saxena,V L Silva,M J Haughey,G E Wood,F Nicolini,H Mirza,I A McNeish,W Huang,E Maniati,T A Graham,M Lockley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 16/K16 antibody [EPR13504]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Western blot - Anti-Cytokeratin 16/K16 antibody [EPR13504] (AB181055)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise