Rabbit Polyclonal Cytokeratin 17 antibody. Cytoskeleton marker. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human KRT17.
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- Nature communications 14:80692023Systemically administered wound-homing peptide accelerates wound healing by modulating syndecan-4 function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoracio Maldonado,Bryan D Savage,Harlan R Barker,Ulrike May,Maria Vähätupa,Rahul K Badiani,Katarzyna I Wolanska,Craig M J Turner,Toini Pemmari,Tuomo Ketomäki,Stuart Prince,Martin J Humphries,Erkki Ruoslahti,Mark R Morgan,Tero A H JärvinenPubMed 38057316
- Cell death & disease 14:7682023Sulforaphane alleviates psoriasis by enhancing antioxidant defense through KEAP1-NRF2 Pathway activation and attenuating inflammatory signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChujun Ma,Chaode Gu,Panpan Lian,Junaid Wazir,Renwei Lu,Binjia Ruan,Lulu Wei,Li Li,Wenyuan Pu,Ziqi Peng,Wentong Wang,Yangyongyi Zong,Zhiqiang Huang,Hongwei Wang,Yan Lu,Zhonglan SuPubMed 38007430
- BMC cancer 23:8112023BCL7B, a SWI/SNF complex subunit, orchestrates cancer immunity and stemness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayaka Higuchi,Yuji Suehiro,Luna Izuhara,Sawako Yoshina,Akira Hirasawa,Shohei MitaniPubMed 37648998
- Genes & genomics 45:1329-13382023Interference KRT17 reverses doxorubicin resistance in triple-negative breast cancer cells by Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiqiong Wu,Wenshuang Ding,Xiaopai Wang,Xiubo Li,Jing YangPubMed 37634232
- International journal of biological sciences 19:3395-34112023Keratin 17 covalently binds to alpha-enolase and exacerbates proliferation of keratinocytes in psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixin Luo,Bingyu Pang,Junfeng Hao,Qingyang Li,Pei Qiao,Chen Zhang,Yaxing Bai,Chunying Xiao,Jiaoling Chen,Dalong Zhi,Ying Liu,Erle Dang,Gang Wang,Bing LiPubMed 37497003
- British journal of pharmacology 180:1748-17652023Isoxazole 9 (ISX9), a small molecule targeting Axin, activates Wnt/β-catenin signalling and promotes hair regrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSapna Sayed,Jiaxing Song,Ling Wang,Tobias Achu Muluh,Boxin Liu,Zhixian Lin,Yun Tang,Zijie Su,Huan Li,Vivian Weiwen Xue,Shanshan Liu,Xianxiong Chen,Guangqian Zhou,Qi Sun,Desheng LuPubMed 36721985
- Cells 12:2023CXXC5 Mediates DHT-Induced Androgenetic Alopecia via PGD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeong Chan Ryu,Jiyeon Park,You-Rin Kim,Sehee Choi,Geon-Uk Kim,Eunhwan Kim,Yumi Hwang,Heejene Kim,Gyoonhee Han,Soung-Hoon Lee,Kang-Yell ChoiPubMed 36831222
- Science advances 9:eabo75552023Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1α signaling and glutamine metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaofeng Wang,Evan Sweren,William Andrews,Yue Li,Junjun Chen,Yingchao Xue,Eric Wier,Martin P Alphonse,Li Luo,Yong Miao,Ruosi Chen,Dongqiang Zeng,Sam Lee,Ang Li,Erika Dare,Dongwon Kim,Nathan K Archer,Sashank K Reddy,Linda Resar,Zhiqi Hu,Elizabeth A Grice,Maureen A Kane,Luis A GarzaPubMed 36598999
- Cell reports 41:1118972022Pyruvate kinase M2 mediates IL-17 signaling in keratinocytes driving psoriatic skin inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlávio P Veras,Gabriel A Publio,Bruno M Melo,Douglas S Prado,Thainá Norbiato,Nerry T Cecilio,Carlos Hiroki,Luis Eduardo A Damasceno,Rebecca Jung,Juliana E Toller-Kawahisa,Timna V Martins,Stella F Assunção,Diogenes Lima,Marcia G Alves,Gabriel V Vieira,Lucas A Tavares,Ana L R Alves-Rezende,Susanne H Karbach,Helder I Nakaya,Thiago M Cunha,Cacilda S Souza,Fernando Q Cunha,Katiuchia U Sales,Ari Waisman,José C Alves-FilhoPubMed 36577385
- Pharmaceutics 14:2022Pyruvate Kinase M2 Promotes Hair Regeneration by Connecting Metabolic and Wnt/β-Catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeong Chan Ryu,You-Rin Kim,Jiyeon Park,Sehee Choi,Won-Ji Ryu,Geon-Uk Kim,Eunhwan Kim,Yumi Hwang,Heejene Kim,Gyoonhee Han,Soung-Hoon Lee,Kang-Yell ChoiPubMed 36559274
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KRT17. The exact immunogen used to generate this antibody is proprietary information. Database link Q04695
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
Alternative names
39.1, Cytokeratin-17, Keratin-17, CK-17, K17, KRT17
Recommended products
Rabbit Polyclonal Cytokeratin 17 antibody. Cytoskeleton marker. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 47 publications. Immunogen corresponding to Synthetic Peptide within Human KRT17.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KRT17. The exact immunogen used to generate this antibody is proprietary information. Database link Q04695
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab53707 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 17 often called K17 or keratin 17 is a type I intermediate filament protein with a molecular weight of approximately 46 kDa. It forms part of the cytoskeleton in epithelial cells and is notably present in the basal cells of epithelial tissues including the skin hair follicles and the lining of internal organs. Due to its specific expression pattern K17 serves as a useful biomarker in various research and clinical settings.
- Biological function summary
The roles of K17 extend beyond mere structural support. This protein contributes to cellular processes such as proliferation tissue repair and the regulation of immune responses. K17 often forms a complex with its partner keratin 6 enabling structural stability and resilience in epithelial cells. Its presence influences the behavior of the cells in skin and other epithelial-lined organs.
- Pathways
K17 participates in key pathways related to skin biology and inflammation notably the Wnt/β-catenin and NF-κB signaling pathways. These pathways connect K17 to cellular processes like growth differentiation and inflammation. In particular keratin 6 is another important protein in these cascades closely interacting with K17 in maintenance and repair processes within epithelial tissues.
- Associated diseases and disorders
K17 has been implicated in psoriasis and certain types of cancer such as cervical and breast cancer. In psoriasis an inflammatory skin condition K17 interacts with pro-inflammatory cytokines contributing to the hyperproliferative state of the skin. Its overexpression serves as a marker in diagnosing and understanding the severity of these disorders highlighting its relationship with keratin 6 which is similarly upregulated in psoriatic lesions and certain tumors. These associations make K17 a significant focus for therapeutic targeting and diagnostic applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)
ab53707 at 1/50 dilution staining Cytokeratin 17 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)
All lanes: Western blot - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707) at 1/500 dilution
Lane 1: HuvEc cell extract
Lane 2: HuvEc cell extract with immunising peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)
ICC/IF image of ab53707 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53707, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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