Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with purified ab183330 at 1/100 (1.6μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human cervix adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) labeling Cytokeratin 17 with purified ab183330 at 1/20 dilution (7.85μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Intracellular flow cytometric analysis of Cytokeratin HeLa cells using ab183330 (green) compare to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 17 with ab183330 at a 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183330).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

Immunohistochemical analysis of paraffin embedded Human cervical adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (AB238808)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).

Flow cytometry overlay histogram showing left HeLa positive cells and right negative MCF7 stained with ab183330 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab183330) (1x 106 in 100μl at 0.008μg/ml (1/262500)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.