Anti-Cytokeratin 17 antibody [SP95] - C-terminal

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative MCF7 stained with ab183330 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab183330) (1x 106 in 100μl at 0.008μg/ml (1/262500)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with purified ab183330 at 1/100 (1.6μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human cervix adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling Cytokeratin 17 with purified ab183330 at 1/20 dilution (7.85 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Flow cytometric analysis of Cytokeratin HeLa cells using ab183330 (green) compare to negative control of rabbit IgG (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human prostate carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human cervical adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling Cytokeratin 17 with ab183330 at 1/100.

• WB

Supplier Data

Western blot - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (AB183330)

All lanes:

Western blot - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330) at 1/25 dilution

All lanes:

HeLa cell lysate

Predicted band size: 48 kDa

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