Mouse Monoclonal Cytokeratin 17 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Tissue preparation containing Krt17 protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98052.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136700.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)")
![Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--western-blot-img142981.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Tissue preparation containing Krt17 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6IFU8
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
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7 products for Alternative Product
Target data
Function
Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
Alternative names
Ka17, Krt17, Cytokeratin-17, Keratin-17, Type I keratin Ka17, CK-17, K17
Recommended products
Mouse Monoclonal Cytokeratin 17 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Tissue preparation containing Krt17 protein.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Tissue preparation containing Krt17 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6IFU8
- Carrier free
- Yes
- Clone number
- SPM560
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
ab212552 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cytokeratin 17 antibody marks basal cell differentiation. Cytokeratin 17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5 and 6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. Cytokeratin 17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-Cytokeratin 17. Also important is that cases of triple negative breast carcinoma with expression of Cytokeratin 17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-Cytokeratin 17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 17 often called K17 or keratin 17 is a type I intermediate filament protein with a molecular weight of approximately 46 kDa. It forms part of the cytoskeleton in epithelial cells and is notably present in the basal cells of epithelial tissues including the skin hair follicles and the lining of internal organs. Due to its specific expression pattern K17 serves as a useful biomarker in various research and clinical settings.
- Biological function summary
The roles of K17 extend beyond mere structural support. This protein contributes to cellular processes such as proliferation tissue repair and the regulation of immune responses. K17 often forms a complex with its partner keratin 6 enabling structural stability and resilience in epithelial cells. Its presence influences the behavior of the cells in skin and other epithelial-lined organs.
- Pathways
K17 participates in key pathways related to skin biology and inflammation notably the Wnt/β-catenin and NF-κB signaling pathways. These pathways connect K17 to cellular processes like growth differentiation and inflammation. In particular keratin 6 is another important protein in these cascades closely interacting with K17 in maintenance and repair processes within epithelial tissues.
- Associated diseases and disorders
K17 has been implicated in psoriasis and certain types of cancer such as cervical and breast cancer. In psoriasis an inflammatory skin condition K17 interacts with pro-inflammatory cytokines contributing to the hyperproliferative state of the skin. Its overexpression serves as a marker in diagnosing and understanding the severity of these disorders highlighting its relationship with keratin 6 which is similarly upregulated in psoriatic lesions and certain tumors. These associations make K17 a significant focus for therapeutic targeting and diagnostic applications.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98052.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 17 with ab212552 at 1 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136700.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 17 with ab212552 at 1 μg/ml.
![Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-17-antibody-spm560-bsa-and-azide-free-ab212552--western-blot-img142981.jpg "Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)")
Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552)
All lanes: Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552) at 1 µg/mL
All lanes: HeLa cell lysate
Predicted band size: 48 kDa
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