AB212552

Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free

Name: Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552) | Abcam
Brand: Abcam Limited
SKU: AB212552
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Cytokeratin 17 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Tissue preparation containing Krt17 protein.

View Alternative Names

Ka17, Krt17, Cytokeratin-17, Keratin-17, Type I keratin Ka17, CK-17, K17

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 17 with ab212552 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 17 with ab212552 at 1 μg/ml.

Supplier Data

Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

All lanes:

Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (ab212552) at 1 µg/mL

All lanes:

HeLa cell lysate

Predicted band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM560

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Tissue preparation containing Krt17 protein. The exact immunogen used to generate this antibody is proprietary information.

Q6IFU8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Cytokeratin 17 antibody marks basal cell differentiation. Cytokeratin 17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5 and 6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. Cytokeratin 17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-Cytokeratin 17. Also important is that cases of triple negative breast carcinoma with expression of Cytokeratin 17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-Cytokeratin 17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab212552 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 17 often called K17 or keratin 17 is a type I intermediate filament protein with a molecular weight of approximately 46 kDa. It forms part of the cytoskeleton in epithelial cells and is notably present in the basal cells of epithelial tissues including the skin hair follicles and the lining of internal organs. Due to its specific expression pattern K17 serves as a useful biomarker in various research and clinical settings.

Biological function summary

The roles of K17 extend beyond mere structural support. This protein contributes to cellular processes such as proliferation tissue repair and the regulation of immune responses. K17 often forms a complex with its partner keratin 6 enabling structural stability and resilience in epithelial cells. Its presence influences the behavior of the cells in skin and other epithelial-lined organs.

Pathways

K17 participates in key pathways related to skin biology and inflammation notably the Wnt/β-catenin and NF-κB signaling pathways. These pathways connect K17 to cellular processes like growth differentiation and inflammation. In particular keratin 6 is another important protein in these cascades closely interacting with K17 in maintenance and repair processes within epithelial tissues.

K17 has been implicated in psoriasis and certain types of cancer such as cervical and breast cancer. In psoriasis an inflammatory skin condition K17 interacts with pro-inflammatory cytokines contributing to the hyperproliferative state of the skin. Its overexpression serves as a marker in diagnosing and understanding the severity of these disorders highlighting its relationship with keratin 6 which is similarly upregulated in psoriatic lesions and certain tumors. These associations make K17 a significant focus for therapeutic targeting and diagnostic applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin : promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.

See full target information Krt17

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 7:145 PubMed23082819

2012

A comprehensive morphological study for basal-like breast carcinomas with comparison to nonbasal-like carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Asli Cakir,Ipek Isik Gonul,Omer Uluoglu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

Western blot - Anti-Cytokeratin 17 antibody [SPM560] - BSA and Azide free (AB212552)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise