AB52948

Anti-Cytokeratin 18 antibody

Name: Anti-Cytokeratin 18 antibody (ab52948) | Abcam
Brand: Abcam Limited
SKU: AB52948
Price: 525 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(29 Publications)

Rabbit Polyclonal Cytokeratin 18 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 29 publications.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (AB52948)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (AB52948)

ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody (AB52948)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody (AB52948)

ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.

Unknown

Western blot - Anti-Cytokeratin 18 antibody (AB52948)

All lanes:

Western blot - Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HR at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Unknown

Western blot - Anti-Cytokeratin 18 antibody (AB52948)

All lanes:

Western blot - Anti-Cytokeratin 18 antibody (ab52948) at 1/250 dilution

All lanes:

Recombinant Human Cytokeratin 18 protein (<a href='/en-us/products/unavailable/recombinant-human-cytokeratin-18-protein-ab73638'>ab73638</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 48 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 65:640-650 PubMed39238338

2024

Luminal progenitor and mature cells are more susceptible than basal cells to radiation-induced DNA double-strand breaks in rat mammary tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Nagata,Mayumi Nishimura,Kazuhiro Daino,Yukiko Nishimura,Yuya Hattori,Ritsuko Watanabe,Daisuke Iizuka,Akinari Yokoya,Keiji Suzuki,Shizuko Kakinuma,Tatsuhiko Imaoka

Nature communications 15:3360 PubMed38637611

2024

CD20/MS4A1 is a mammalian olfactory receptor expressed in a subset of olfactory sensory neurons that mediates innate avoidance of predators.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Ching Jiang,Sung Jin Park,I-Hao Wang,Daniel M Bear,Alexandra Nowlan,Paul L Greer

eLife 11: PubMed36111787

2022

Sociosexual behavior requires both activating and repressive roles of AP-2ε in vomeronasal sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Lin,Tyler A Mitchell,Megan Rothstein,Alison Pehl,Ed Zandro M Taroc,Raghu R Katreddi,Katherine E Parra,Damian G Zuloaga,Marcos Simoes-Costa,Paolo Emanuele Forni

ACS biomaterials science & engineering 8:3819-3830 PubMed35994527

2022

Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha G Zambuto,Ishita Jain,Kathryn B H Clancy,Gregory H Underhill,Brendan A C Harley

Translational oncology 20:101398 PubMed35339890

2022

PLAG alleviates cisplatin-induced cachexia in lung cancer implanted mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guen Tae Kim,Eun Young Kim,Su-Hyun Shin,Hyowon Lee,Se Hee Lee,Kaapjoo Park,Ki-Young Sohn,Sun Young Yoon,Jae Wha Kim

Stem cell research 52:102180 PubMed33556820

2021

Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (Ɛ2/Ɛ3/Ɛ4) and knock-out for the study of APOE biology in health and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Schmid,Bjørn Holst,Christian Clausen,Lamiaa Bahnassawy,Peter Reinhardt,Margot H M Bakker,Eva Díaz-Guerra,Carlos Vicario,Pamela V Martino-Adami,Michaela Thoenes,Alfredo Ramirez,Klaus Fliessbach,Clara Grezella,Oliver Brüstle,Michael Peitz,Andreas Ebneth,Alfredo Cabrera-Socorro

Scientific reports 10:18246 PubMed33106532

2020

Mesoscopic 3D imaging of pancreatic cancer and Langerhans islets based on tissue autofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Max Hahn,Christoffer Nord,Oskar Franklin,Tomas Alanentalo,Martin Isaksson Mettävainio,Federico Morini,Maria Eriksson,Olle Korsgren,Malin Sund,Ulf Ahlgren

Radiation and environmental biophysics 59:711-721 PubMed32996008

2020

The effect of radiation on the ability of rat mammary cells to form mammospheres.

Applications

Unspecified application

Species

Unspecified reactive species

Ayaka Hosoki,Mari Ogawa,Yukiko Nishimura,Mayumi Nishimura,Kazuhiro Daino,Shizuko Kakinuma,Yoshiya Shimada,Tatsuhiko Imaoka

Cells 9: PubMed32707902

2020

Fibroblast Growth Factor-14 Acts as Tumor Suppressor in Lung Adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Kati Turkowski,Frederik Herzberg,Stefan Günther,David Brunn,Andreas Weigert,Michael Meister,Thomas Muley,Mark Kriegsmann,Marc A Schneider,Hauke Winter,Michael Thomas,Friedrich Grimminger,Werner Seeger,Soni Savai Pullamsetti,Rajkumar Savai

PLoS pathogens 16:e1008304 PubMed32069333

2020

A three-dimensional intestinal tissue model reveals factors and small regulatory RNAs important for colonization with Campylobacter jejuni.

Applications

Unspecified application

Species

Unspecified reactive species

Mona Alzheimer,Sarah L Svensson,Fabian König,Matthias Schweinlin,Marco Metzger,Heike Walles,Cynthia M Sharma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 18 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (AB52948)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody (AB52948)

Western blot - Anti-Cytokeratin 18 antibody (AB52948)

Western blot - Anti-Cytokeratin 18 antibody (AB52948)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

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