Anti-Cytokeratin 18 antibody [C-04]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (AB668)

Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labelling cytokeratin-18 with ab668 at 10μg/ml concentration.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (AB668)

Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).

The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (AB668)

Intracellular flow cytometric analysis (intracellular staining) of HeLa cells (human epithelial cell line from cervix adenocarcinoma cell suspension)stained with 0.6μg/ml ab668 (in red), and unstained HeLa cells (black).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (AB668)

Flow cytometric pattern (intracellular staining) of HeLa cells (Human epithelial cell line from cervix adenocarcinoma cell suspension) stained with ab668 (0.6 μg/ml concentration)