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Mouse Monoclonal Cytokeratin 18 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (AB7797), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (AB7797), expandable thumbnail
  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (AB7797), expandable thumbnail
  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (AB7797), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (AB7797), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

-

Tested
Tested

Species

Human

Dilution info

6.00000-16.00000 µg/mL

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 18 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 18 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

DC 10

Purification technique

Affinity purification Protein A

Specificity

Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.

Concentration
Loading...
Purification notes

Purified from TCS

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 μg/ml; orange).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml

  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/mL

    Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)

    Lane 2: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)

    Lane 3: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)

    Lane 4: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)

    Secondary

    All lanes: IRDye800-conjugated anti-mouse secondary antibody.

    Predicted band size: 48 kDa

  • Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/mL

    Predicted band size: 48 kDa

    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

    Flow cytometry analysis (intracellular staining) of MCF-7 cells (red-filled) stained using ab7797 anti-cytokeratin 18 purified antibody (concentration in sample 0,6 μg/ml, GAM APC). Isotype control: Caco-2 cells (black-dashed).

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Product protocols

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