Anti-Cytokeratin 18 antibody [DC 10]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 μg/ml; orange).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

Flow cytometry analysis (intracellular staining) of MCF-7 cells (red-filled) stained using ab7797 anti-cytokeratin 18 purified antibody (concentration in sample 0,6 μg/ml, GAM APC). Isotype control : Caco-2 cells (black-dashed).

• WB

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/mL

Lane 1:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)

Secondary

All lanes:

IRDye800-conjugated anti-mouse secondary antibody.

Predicted band size: 48 kDa

false

• WB

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [DC 10] (AB7797)

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 48 kDa

Observed band size: 40 kDa

false