AB181597

Anti-Cytokeratin 18 antibody [EPR17347]

Name: Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) | Abcam
Brand: Abcam Limited
SKU: AB181597
Price: 215 USD
Availability: InStock
Rating: 5 (3 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(3 Reviews)

(32 Publications)

Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) is a rabbit monoclonal antibody detecting Cytokeratin 18 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of Human liver. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tumor cells of Human colon cancer. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of mouse liver. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tubules of rat kidney. Counter stained with Hematoxylin.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The observed MW is consistent with what has been described in the literature (PMID : 9298992).

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution

Lane 1:

Mouse colon lysate at 10 µg

Lane 2:

Rat lung lysate at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The generation of the 22kDa fragment was inhibited when the caspase 3 activity was blocked by the caspase inhibitor, Z-VAD-FMK.

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate treated with 50uM Z-VAD-FMK for 4 hours at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The 22 kDa cytokeratin 18 fragment was generated (lane 2) in the staurosporine-treated lysate that contained the active caspase 3.

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 22 kDa,45 kDa,48 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The observed MW is consistent with what has been described in the literature (PMID : 9298992).

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution

All lanes:

Human fetal skin lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

Exposure time: 2min

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The observed MW is consistent with what has been described in the literature (PMID : 9298992).

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/2000 dilution

All lanes:

F9 (Mouse embyro testicular cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

5% NFDM/TBST : Blocking and diluting buffer.

The observed MW is consistent with what has been described in the literature (PMID : 9298992).

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

Exposure time: 5s

Carrier free

Anti-Cytokeratin 18 antibody [EPR17347] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17347

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Cytokeratin 18?
Anti-Cytokeratin 18 [EPR17347] (ab181597) specifically detects a band for Cytokeratin 18 (UniProt: P05784) at a molecular weight of 47kDa.

Trusted by the scientific community
Anti-Cytokeratin 18 [EPR17347] (ab181597) was first used in a scientific publication in 2015 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR17347] also available for your convenience: ab181597, Carrier free - ab240254

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40728884

2025

Living human lung slices for ex vivo modelling of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siavash Mansouri,Annika Karger,Clemens Ruppert,Marc A Schneider,Andreas Weigert,Rajender Nandigama,Blerina Aliraj,Lisa Strotmann,Anoop V Cherian,Diethard Pruefer,Peter Dorfmuller,Ludger Fink,Ibrahim Alkoudmani,Stefan Gattenlöhner,Bastian Eul,Andre Althoff,Peter Kleine,Hauke Winter,Andreas Guenther,Hossein-Ardeschir Ghofrani,Soni S Pullamsetti,Friedrich Grimminger,Werner Seeger,Rajkumar Savai

Cell death discovery 11:139 PubMed40180904

2025

Integrative insights into the role of CAV1 in ketogenic diet and ferroptosis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Liang,Ruofei Tian,Ting Li,Hao Wang,Yifei Qin,Meirui Qian,Jing Fan,Dan Wang,Hong-Yong Cui,Jianli Jiang

Cell communication and signaling : CCS 23:148 PubMed40119472

2025

Platelet glycoprotein VI promotes folic acid-induced acute kidney injury through interaction with tubular epithelial cell-derived galectin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Wei Guo,Qi Luo,Meng Lu,Xiang-Bin Zeng,Yu-Min Zhang,Yue-Ling Lin,Xu-Ran Guo,Rong Ma,Zhang-Yin Ming

International journal of molecular sciences 25: PubMed39595946

2024

Linking Antibodies Against Apolipoprotein A-1 to Metabolic Dysfunction-Associated Steatohepatitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Pagano,Emmanuel Somm,Catherine Juillard,Nicolas Liaudet,Frédérique Ino,Johan Ferrari,Vincent Braunersreuther,François R Jornayvaz,Nicolas Vuilleumier

Nature materials 23:1748-1758 PubMed39134648

2024

Substrates mimicking the blastocyst geometry revert pluripotent stem cell to naivety.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Xu,Weiwei Wang,Yue Liu,Johan Bäckemo,Matthias Heuchel,Wei Wang,Yan Nie,Imran Iqbal,Karl Kratz,Andreas Lendlein,Nan Ma

Cancer research 84:3235-3249 PubMed39024552

2024

CCN6 Suppresses Metaplastic Breast Carcinoma by Antagonizing Wnt/β-Catenin Signaling to Inhibit EZH2-Driven EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Gonzalez,Bryce Brophy,Ahmad Eido,Adele E Leonetti,Sabra I Djomehri,Giuseppina Augimeri,Nicholas J Carruthers,Raymond G Cavalcante,Francesca Giordano,Sebastiano Andò,Alexey I Nesvizhskii,Eric R Fearon,Celina G Kleer

Oncogene 43:1178-1189 PubMed38396293

2024

miR-147b mediated suppression of DUSP8 promotes lung cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kati Turkowski,Frederik Herzberg,Stefan Günther,Andreas Weigert,Tamara Haselbauer,Ludger Fink,David Brunn,Friedrich Grimminger,Werner Seeger,Holger Sültmann,Thorsten Stiewe,Soni S Pullamsetti,Rajkumar Savai

Journal of translational medicine 21:553 PubMed37592347

2023

Rutaecarpine induces the differentiation of triple-negative breast cancer cells through inhibiting fumarate hydratase.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Lei,Yujia Pan,Rui Gao,Bin He,Zifeng Wang,Xinxing Lei,Zijian Zhang,Na Yang,Min Yan

European journal of histochemistry : EJH 67: PubMed36546419

2023

Differential antigen expression between human apocrine sweat glands and eccrine sweat glands.

Applications

Unspecified application

Species

Unspecified reactive species

Manxiu Cao,Lei Zhang,Jiaqi Chen,Cangyu Wang,Junhong Zhao,Xiang Liu,Yongjing Yan,Yue Tang,Zixiu Chen,Haihong Li

Biomolecules & biomedicine 23:437-449 PubMed36336986

2022

Promotive role of IRF7 in ferroptosis of colonic epithelial cells in ulcerative colitis by the miR-375-3p/SLC11A2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zepeng Chen,Qinglong Gu,Ruichao Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 18 antibody [EPR17347]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Product promise