Name: Anti-Cytokeratin 18 antibody [EPR17347]
SKU: ab181597
Rating: 5 (3 reviews)

Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) is a rabbit monoclonal antibody that is used to detect Cytokeratin 18 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt	WB	IHC-P
Human	Not recommended	Not recommended	Tested	Tested
Mouse	Not recommended	Not recommended	Tested	Tested
Rat	Not recommended	Not recommended	Expected	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -
Species Human	Dilution info 1/2000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/800	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/800	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/800	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information KRT18

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17347
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human, Mouse, Rat samples.

What is the molecular weight of Cytokeratin 18?

Anti-Cytokeratin 18 [EPR17347] (ab181597) specifically detects a band for Cytokeratin 18 (UniProt: P05784) at a molecular weight of 47kDa.

Recommended positive controls

WB: Mouse colon, rat lung, mouse kidney, human fetal skin and mouse spleen tissue lysates, HeLa and F9 cell lysates. IHC-P: Human liver and colonic adenocarcinoma, mouse liver and rat kidney tissue.

Trusted by the scientific community

Anti-Cytokeratin 18 [EPR17347] (ab181597) was first used in a scientific publication in 2015 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EPR17347] also available for your convenience:
ab181597, Carrier free - ab240254

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tumor cells of Human colon cancer. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of Human liver. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of mouse liver. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tubules of rat kidney. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution
Lane 1: Mouse colon lysate at 10 µg
Lane 2: Rat lung lysate at 10 µg
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Exposure time: 30s
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1: Mouse kidney lysate at 10 µg
Lane 2: Mouse spleen lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Exposure time: 5s
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution
All lanes: Human fetal skin lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Exposure time: 2min
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/2000 dilution
All lanes: F9 (Mouse embyro testicular cancer cell line) whole cell lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Exposure time: 3min
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The 22 kDa cytokeratin 18 fragment was generated (lane 2) in the staurosporine-treated lysate that contained the active caspase 3.
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1: Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HeLa whole cell lysate treated with 1uM staurosporine for 4 hours at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 22 kDa, 45 kDa, 48 kDa
Exposure time: 15s
Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597)
5% NFDM/TBST: Blocking and diluting buffer.
The generation of the 22kDa fragment was inhibited when the caspase 3 activity was blocked by the caspase inhibitor, Z-VAD-FMK.
All lanes: Western blot - Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1: Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2: HeLa whole cell lysate treated with 50uM Z-VAD-FMK for 4 hours at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa, 48 kDa
Exposure time: 2s