AB9217

Anti-Cytokeratin 18 antibody [RGE53]

Name: Anti-Cytokeratin 18 antibody [RGE53] (ab9217) | Abcam
Brand: Abcam Limited
SKU: AB9217
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Mouse Monoclonal Cytokeratin 18 antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Pig, Zebrafish samples. Cited in 8 publications. Immunogen corresponding to Full Length Protein corresponding to Human KRT18.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

10 Images

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry on frozen section of human kidney epithelium.

Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry on frozen section of human kidney epithelium

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry on frozen section of human colon

Unknown

Western blot - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

All lanes:

Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

true

Exposure time: 30s

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunofluorescence staining of 1 month old zebrafish embryo.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone : Methanol 1 : 1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry on frozen section of swine liver hepatocytes

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunofluorescence staining images of 9 day old zebrafish embryos.
ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone : Methanol 1 : 1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RGE53

Isotype

IgG1

Carrier free

Reacts with

Human, Pig, Zebrafish

Applications

WB, IHC-Fr, Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human KRT18. The exact immunogen used to generate this antibody is proprietary information.

P05783

Specificity

This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Hamster": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37446098

2023

Glypican-3 (GPC-3) Structural Analysis and Cargo in Serum Small Extracellular Vesicles of Hepatocellular Carcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Montalbano Mauro,Perricone Ugo,Zachary Walton,Shirafkan Ali,Cristiana Rastellini,Luca Cicalese

PloS one 11:e0153613 PubMed27074018

2016

Modeling of Hepatocytes Proliferation Isolated from Proximal and Distal Zones from Human Hepatocellular Carcinoma Lesion.

Applications

IHC-P, WB

Species

Human, Human

Mauro Montalbano,Giuseppe Curcurù,Ali Shirafkan,Renza Vento,Cristiana Rastellini,Luca Cicalese

International journal of oncology 48:1205-17 PubMed26717856

2016

Transformation of primary human hepatocytes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mauro Montalbano,Cristiana Rastellini,Xiaofu Wang,Tiziana Corsello,Mahmoud A Eltorky,Renza Vento,Luca Cicalese

Developmental biology 391:17-31 PubMed24727670

2014

Tbx18 is essential for normal development of vasculature network and glomerular mesangium in the mammalian kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Jinshu Xu,Xuguang Nie,Xiaoqiang Cai,Chen-Leng Cai,Pin-Xian Xu

Biochemical and biophysical research communication 390:931-6 PubMed19852942

2009

Cell-to-cell contact induces human adipose tissue-derived stromal cells to differentiate into urothelium-like cells in vitro.

Applications

ICC/IF

Species

Human

Jie Liu,Jian Huang,Tianxin Lin,Caixia Zhang,Xinbao Yin

The American journal of pathology 136:641-55 PubMed1690512

1990

Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

F Ramaekers,C van Niekerk,L Poels,E Schaafsma,A Huijsmans,H Robben,G Schaart,P Vooijs

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 4:300-13 PubMed2417968

1985

Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix.

Applications

Unspecified application

Species

Unspecified reactive species

J J Puts,O Moesker,P Kenemans,G P Vooijs,F C Ramaekers

Laboratory investigation; a journal of technical methods and pathology 49:353-61 PubMed6193333

1983

Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology.

Applications

Unspecified application

Species

Unspecified reactive species

F Ramaekers,A Huysmans,O Moesker,A Kant,P Jap,C Herman,P Vooijs

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 18 antibody [RGE53]

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Western blot - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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