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Mouse Monoclonal Cytokeratin 18 antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Pig, Zebrafish samples. Cited in 11 publications. Immunogen corresponding to Full Length Protein corresponding to Human Keratin, type I cytoskeletal 18.


Images

Western blot - Anti-Cytokeratin 18 antibody [RGE53] (AB9217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (AB9217), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Human Keratin, type I cytoskeletal 18. The exact immunogen used to generate this antibody is proprietary information. Database link P05783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-Fr
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Hamster
Predicted
Predicted
Predicted
Pig
Expected
Expected
Tested
Rabbit
Predicted
Predicted
Predicted
Zebrafish
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Pig, Zebrafish

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Hamster, Dog

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Pig, Zebrafish

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Hamster, Dog

Dilution info

-

Notes

-

Tested
Tested

Species

Pig

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Species

Zebrafish

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Species

Human

Dilution info

1/100.00000 - 1/200.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Rabbit, Chicken, Hamster, Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 18 antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human, Pig, Zebrafish samples. Cited in 11 publications. Immunogen corresponding to Full Length Protein corresponding to Human Keratin, type I cytoskeletal 18.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Human Keratin, type I cytoskeletal 18. The exact immunogen used to generate this antibody is proprietary information. Database link P05783
Clone number

RGE53

Purification technique

Affinity purification Protein G

Specificity

This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

Product promise

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10 product images

  • Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

    Exposure time: 30s

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunohistochemistry on frozen section of human kidney epithelium.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunofluorescence staining of 1 month old zebrafish embryo.

  • Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunohistochemistry on frozen section of human kidney epithelium

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunohistochemistry on frozen section of human colon

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunohistochemistry on frozen section of swine liver hepatocytes

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