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Rabbit Recombinant Multiclonal Cytokeratin 18 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (AB315097), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (AB315097), expandable thumbnail
  • Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (AB315097), expandable thumbnail
  • Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (AB315097), expandable thumbnail
  • Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (AB315097), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Tested
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

Recommended products

Rabbit Recombinant Multiclonal Cytokeratin 18 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
RM1051
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for Rat ICC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315097 is the carrier-free version of Anti-Cytokeratin 18 antibody [RM1051] ab315096.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Molt-4 (human lymphoblastic leukemia T lymphoblast, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 dilution (0.1 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: Molt-4.

  • Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Cytokeratin 18 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

    Lane 2: Anti-Cytokeratin 18 antibody [RM1051] ab315096 IP in Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cytokeratin 18 antibody [RM1051] ab315096 in Hepa1-6 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] (Anti-Cytokeratin 18 antibody [RM1051] ab315096) at 1/30 dilution

    All lanes: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Skeletal muscle.

    The observed MW is consistent with what has been described in the literature (PMID:9298992).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1: 26 seconds; Lanes 2-7: 180 seconds.

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [RM1051] (Anti-Cytokeratin 18 antibody [RM1051] ab315096) at 1/1000 dilution

    Lane 1: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Mouse colon tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Lane 7: AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45, 48 kDa, 48 kDa, 36 kDa

  • Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Cytokeratin 18 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell lysate with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-Cytokeratin 18 antibody [RM1051] ab315096 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cytokeratin 18 antibody [RM1051] ab315096 in A431 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 18 antibody [RM1051] (Anti-Cytokeratin 18 antibody [RM1051] ab315096) at 1/30 dilution

    All lanes: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Skeletal muscle.

    Generation of the 22kDa fragment can be induced by staurosporine treatment (lane 1-2) (PMID: 34322741 and PMID:9298992).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 8 seconds; Lanes 3-5: 1 second.

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [RM1051] (Anti-Cytokeratin 18 antibody [RM1051] ab315096) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 2uM staurosporine for 24 hours whole cell lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Lane 5: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 22 kDa, 48 kDa, 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/50 (9.48 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in Hepa1-6 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/50 (9.48 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in A431 cell line. Negative control: MOLT-4.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human cerebrum. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat stomach. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse stomach. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human gastric cancer. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human ovarian cancer. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human stomach. The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cytokeratin 18 with Anti-Cytokeratin 18 antibody [RM1051] ab315096 at 1/500 (0.948 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon.The section was incubated with Anti-Cytokeratin 18 antibody [RM1051] ab315096 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 antibody [RM1051] - BSA and Azide free (ab315097)

    This data was developed using Anti-Cytokeratin 18 antibody [RM1051] ab315096, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MOLT-4 (PMID: 2473821)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Cytokeratin 18 antibody [RM1051] (Anti-Cytokeratin 18 antibody [RM1051] ab315096) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 7 µg

    Lane 2: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 48 kDa, 36 kDa

    Exposure time: 4s

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