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Rabbit Polyclonal Cytokeratin 18 phospho S52 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 18 phospho S52.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 (phospho S52) antibody (AB63393), expandable thumbnail
  • Western blot - Anti-Cytokeratin 18 (phospho S52) antibody (AB63393), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Keratin, type I cytoskeletal 18 phospho S52. The exact immunogen used to generate this antibody is proprietary information. Database link P05783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

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Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Rabbit Polyclonal Cytokeratin 18 phospho S52 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 18 phospho S52.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Keratin, type I cytoskeletal 18 phospho S52. The exact immunogen used to generate this antibody is proprietary information. Database link P05783
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)

    Immunofluorescence analysis of HeLa cells, using ab63393 at a 1:500 dilution.
    Left image untreated.
    Right image treated with phospho peptide.

  • Western blot - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393), expandable thumbnail

    Western blot - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393)

    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

    All lanes: Western blot - Anti-Cytokeratin 18 (phospho S52) antibody (ab63393) at 1/500 dilution

    Lane 1: Extracts from HepG2 cells

    Lane 2: Extracts from HepG2 cells plus phospho peptide

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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