AB194399

Anti-Cytokeratin 19 antibody [A53-B]

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(5 Publications)

Mouse Monoclonal Cytokeratin 19 antibody. Suitable for ICC, WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing KRT19 protein.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Cytokeratin 19 with ab194399 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Lab

Immunocytochemistry - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

ab194399 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab194399 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Supplier Data

Immunocytochemistry - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Cytokeratin 19 with ab194399 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (cyan). The nuclear counterstain is Reddot (red).

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (ab194399) at 2 µg/mL

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (ab194399) at 2 µg/mL

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A53-B

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Cell preparation containing KRT19 protein. The exact immunogen used to generate this antibody is proprietary information.

P08727

Specificity

This antibody reacts with the rod domain of human Cytokeratin 19, a polypeptide of 40 kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "Signal can be observed in cells fixed with either methanol or paraformaldehyde.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "Signal can be observed in cells fixed with either methanol or paraformaldehyde.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Ferret": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "Signal can be observed in cells fixed with either methanol or paraformaldehyde.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

This antibody is made up of IgG2a, kappa (A53-B/A2) and IgG1, kappa (BA17).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from bioreactor concentrates.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114286 PubMed38796854

2024

CRACD loss induces neuroendocrine cell plasticity of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bongjun Kim,Shengzhe Zhang,Yuanjian Huang,Kyung-Pil Ko,Youn-Sang Jung,Jinho Jang,Gengyi Zou,Jie Zhang,Sohee Jun,Kee-Beom Kim,Kwon-Sik Park,Jae-Il Park

Frontiers in medicine 8:683506 PubMed34336890

2021

Hepatic BRD4 Is Upregulated in Liver Fibrosis of Various Etiologies and Positively Correlated to Fibrotic Severity.

Applications

Unspecified application

Species

Unspecified reactive species

Cichun Wu,Da Cheng,Yanghui Peng,Ying Li,Chunyan Fu,Ying Wang,Lei Fu,Shifang Peng,Xin Ni

Cell metabolism 32:1028-1040.e4 PubMed33207245

2020

SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 Are Expressed in the Microvasculature and Ducts of Human Pancreas but Are Not Enriched in β Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Katie C Coate,Jeeyeon Cha,Shristi Shrestha,Wenliang Wang,Luciana Mateus Gonçalves,Joana Almaça,Meghan E Kapp,Maria Fasolino,Ashleigh Morgan,Chunhua Dai,Diane C Saunders,Rita Bottino,Radhika Aramandla,Regina Jenkins,Roland Stein,Klaus H Kaestner,Golnaz Vahedi,Marcela Brissova,Alvin C Powers

bioRxiv : the preprint server for biology : PubMed33106804

2020

SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 are Expressed in the Pancreas but are Not Enriched in Islet Endocrine Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Journal of cellular and molecular medicine 24:1128-1140 PubMed31755222

2019

Fibroblast growth factor-2/platelet-derived growth factor enhances atherosclerotic plaque stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mao,Xiao Qiong Liu,Yu Song,Chun Gang Zhai,Xing Li Xu,Lei Zhang,Yun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 19 antibody [A53-B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Immunocytochemistry - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Immunocytochemistry - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Western blot - Anti-Cytokeratin 19 antibody [A53-B] (AB194399)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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