Rabbit Polyclonal Cytokeratin 19 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 19.
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- Cells 10:2021Selective Targeting of Cancer-Associated Fibroblasts by Engineered H-Ferritin Nanocages Loaded with Navitoclax.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeopoldo Sitia,Arianna Bonizzi,Serena Mazzucchelli,Sara Negri,Cristina Sottani,Elena Grignani,Maria Antonietta Rizzuto,Davide Prosperi,Luca Sorrentino,Carlo Morasso,Raffaele Allevi,Marta Sevieri,Filippo Silva,Marta Truffi,Fabio CorsiPubMed 33562504
- PLoS neglected tropical diseases 13:e00068432019Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Min Kim et. al.PubMed 30943209
- Hepatology communications 3:277-2922019Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNataliya Razumilava,Junya Shiota,Nureen H Mohamad Zaki,Ramon Ocadiz-Ruiz,Christine M Cieslak,Kais Zakharia,Benjamin L Allen,Gregory J Gores,Linda C Samuelson,Juanita L MerchantPubMed 30766964
- Hepatology (Baltimore, Md.) 69:622-6382019Neoplastic Transformation of the Peribiliary Stem Cell Niche in Cholangiocarcinoma Arisen in Primary Sclerosing Cholangitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuido Carpino et. al.PubMed 30102768
- Cancer research 78:4253-42692018Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria Quaranta et. al.PubMed 29789416
- Oncotarget 7:33165-782016Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNami Miura,Masahiro Kamita,Takanori Kakuya,Yutaka Fujiwara,Koji Tsuta,Hideaki Shiraishi,Fumitaka Takeshita,Takahiro Ochiya,Hirokazu Shoji,Wilber Huang,Yuichiro Ohe,Tesshi Yamada,Kazufumi HondaPubMed 27121206
- International journal of molecular medicine 37:1263-732016Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.
- Cell cycle (Georgetown, Tex.) 14:3498-5052015Direct reprogramming of human fibroblasts into sweat gland-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiliang Zhao,Mengyao Xu,Meng Wu,Kui Ma,Mengli Sun,Xiaocheng Tian,Cuiping Zhang,Xiaobing FuPubMed 26566868
- Cell death & disease 5:e14922014Nuclear iASPP may facilitate prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE V Morris et. al.PubMed 25341046
- Current eye research 40:30-92014The impact of storage temperature on the morphology, viability, cell number and metabolism of cultured human conjunctival epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJon R Eidet,Øygunn A Utheim,Rakibul Islam,Torstein Lyberg,Edvard B Messelt,Darlene A Dartt,Tor P UtheimPubMed 24750037
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Keratin, type I cytoskeletal 19. The exact immunogen used to generate this antibody is proprietary information. Database link P08727
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Alternative names
Cytokeratin-19, Keratin-19, CK-19, K19, KRT19
Recommended products
Rabbit Polyclonal Cytokeratin 19 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 19.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Keratin, type I cytoskeletal 19. The exact immunogen used to generate this antibody is proprietary information. Database link P08727
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
- Biological function summary
CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.
- Pathways
CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.
- Associated diseases and disorders
CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody (ab53119)
ab53119 at 1/50 dilution staining Cytokeratin 19 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Western blot - Anti-Cytokeratin 19 antibody (ab53119)
All lanes: Western blot - Anti-Cytokeratin 19 antibody (ab53119) at 1/300 dilution
Lane 1: LOVO cell extract
Lane 2: LOVO cell extract with immunising peptide
Predicted band size: 44 kDa
Observed band size: 44 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody (ab53119)
ICC/IF image of ab53119 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53119, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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