AB53119

Anti-Cytokeratin 19 antibody

Name: Anti-Cytokeratin 19 antibody (ab53119) | Abcam
Brand: Abcam Limited
SKU: AB53119
Price: 545 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(12 Publications)

Rabbit Polyclonal Cytokeratin 19 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human KRT19.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody (AB53119)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody (AB53119)

ab53119 at 1/50 dilution staining Cytokeratin 19 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody (AB53119)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody (AB53119)

ICC/IF image of ab53119 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53119, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Cytokeratin 19 antibody (AB53119)

All lanes:

Western blot - Anti-Cytokeratin 19 antibody (ab53119) at 1/300 dilution

Lane 1:

LOVO cell extract

Lane 2:

LOVO cell extract with immunising peptide

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KRT19. The exact immunogen used to generate this antibody is proprietary information.

P08727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cureus 16:e72365 PubMed39583399

2024

Detection of Protein Markers From Blood Samples of Cervical Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Shahana Sharmin,Maha Jamiruddin,Mohd Raeed Jamiruddin,Abul Bashar Mir Md K Islam,Chowdhury R Ahsan,Mahmuda Yasmin

Cells 10: PubMed33562504

2021

Selective Targeting of Cancer-Associated Fibroblasts by Engineered H-Ferritin Nanocages Loaded with Navitoclax.

Applications

Unspecified application

Species

Unspecified reactive species

Leopoldo Sitia,Arianna Bonizzi,Serena Mazzucchelli,Sara Negri,Cristina Sottani,Elena Grignani,Maria Antonietta Rizzuto,Davide Prosperi,Luca Sorrentino,Carlo Morasso,Raffaele Allevi,Marta Sevieri,Filippo Silva,Marta Truffi,Fabio Corsi

Frontiers in immunology 11:297 PubMed32174917

2020

Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Ireland,Teifion Luckett,Michael C Schmid,Ainhoa Mielgo

PLoS neglected tropical diseases 13:e0006843 PubMed30943209

2019

Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Min Kim,Young Mee Bae,Min-Ho Choi,Sung-Tae Hong

Hepatology communications 3:277-292 PubMed30766964

2019

Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nataliya Razumilava,Junya Shiota,Nureen H Mohamad Zaki,Ramon Ocadiz-Ruiz,Christine M Cieslak,Kais Zakharia,Benjamin L Allen,Gregory J Gores,Linda C Samuelson,Juanita L Merchant

Hepatology (Baltimore, Md.) 69:622-638 PubMed30102768

2019

Neoplastic Transformation of the Peribiliary Stem Cell Niche in Cholangiocarcinoma Arisen in Primary Sclerosing Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Guido Carpino,Vincenzo Cardinale,Trine Folseraas,Diletta Overi,Krzysztof Grzyb,Daniele Costantini,Pasquale Bartolomeo Berloco,Sabina Di Matteo,Tom Hemming Karlsen,Domenico Alvaro,Eugenio Gaudio

Cancer research 78:4253-4269 PubMed29789416

2018

Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Quaranta,Carolyn Rainer,Sebastian R Nielsen,Meirion L Raymant,Muhammad S Ahmed,Dannielle D Engle,Arthur Taylor,Trish Murray,Fiona Campbell,Daniel H Palmer,David A Tuveson,Ainhoa Mielgo,Michael C Schmid

Oncotarget 7:33165-78 PubMed27121206

2016

Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.

Applications

Unspecified application

Species

Unspecified reactive species

Nami Miura,Masahiro Kamita,Takanori Kakuya,Yutaka Fujiwara,Koji Tsuta,Hideaki Shiraishi,Fumitaka Takeshita,Takahiro Ochiya,Hirokazu Shoji,Wilber Huang,Yuichiro Ohe,Tesshi Yamada,Kazufumi Honda

International journal of molecular medicine 37:1263-73 PubMed26986690

2016

Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.

Applications

WB, IHC-P

Species

Human, Human

Shaowei Yang,Yexiao Sun,Zhijun Geng,Kui Ma,Xiaoyan Sun,Xiaobing Fu

Cell cycle (Georgetown, Tex.) 14:3498-505 PubMed26566868

2015

Direct reprogramming of human fibroblasts into sweat gland-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiliang Zhao,Mengyao Xu,Meng Wu,Kui Ma,Mengli Sun,Xiaocheng Tian,Cuiping Zhang,Xiaobing Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 19 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody (AB53119)

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody (AB53119)

Western blot - Anti-Cytokeratin 19 antibody (AB53119)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise