Anti-Cytokeratin 19 antibody [BA16]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [BA16] (AB20210)

IHC image of Cytokeratin 19 staining in human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20210, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ICC

AbReview15922****

Immunocytochemistry - Anti-Cytokeratin 19 antibody [BA16] (AB20210)

ab20210 at 1/100 dilution staining Cytokeratin 19 breast cancer cell line by immunocytochemistry. Sections were methanol fixed prior to blocking in 10% serum for 5 minutes and then incubated with ab20210, for 1 hour at 25°C. Ultravision ONE HRP rabbit polyclonal to mouse Ig was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Rene Villadsen

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [BA16] (AB20210)

ab20210 staining Cytokeratin 19 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab20210 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

Lab

Western blot - Anti-Cytokeratin 19 antibody [BA16] (AB20210)

All lanes:

Anti-Cytokeratin 19 antibody [BA16] (ab20210) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 3:

Human tonsil normal tissue lysate - total protein (<a href='/en-us/products/unavailable/human-tonsil-normal-tissue-lysate-total-protein-ab29615'>ab29615</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 40 kDa,44 kDa

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Exposure time: 1min