Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

Flow cytometry overlay histogram showing MCF7 positive cells (left) and SH-SY5Y negative cells (right) stained with ab52625 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab52625) (1x 10⁶ in 100μl at 0.04μg/ml (1/50,000)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in MCF7 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• mIHC

Collaborator

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Red; TG700N), anti-CD163 (ab182422; Brown; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Violet; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain. The inset image shows the separate CD68 signal.

The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

This image is courtesy of TissueGnostics Asia Pacific Limited

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurified ab52625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52625, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

ab52625 staining Cytokeratin 19 in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluorr^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 488). Please refer to ab192643 for protocol details.

ab192643 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Unpurified ab52625 showing positive staining in Breast carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Immunohistochemical staining of paraffin-embedded human skin with purified ab52625 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

ab52625 staining KRT19 in MCF7 cells, with negative expression in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab52625 at 2 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown. This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions. This data was developed using the same antibody clone in a different buffer formulation (ab52625).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining Carboxypeptidase A with ab317620 at a 1/1000 dilution, ab322202 anti-Inhibin beta E chain used at 1/2000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-VAP1 (green; Opal^™520), anti-Inhibin beta E chain (magenta; Opal^™690) and anti-Cytokeratin 19 (gray; Opal^™570) on human liver. Panel B : anti-VAP1 staining endothelium in human liver.
Panel C : anti-Inhibin beta E chain staining hepatocytes in human liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317620, ab322202 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Fluorescence multiplex immunohistochemical analysis of human liver tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of ab195872 anti-Cytokeratin 19 stained on branch of bile ducts (magenta; Opal™690) at 1 : 8000 ( 0.127 μg/ml) [Panel B], ab275376 anti-Factor VIII stained on endothelial cells (red; Opal™570) at 1 : 1000 ( 0.457 μg/ml) [Panel C], and ab150301 anti-alpha smooth muscle Actin stained on smooth muscles (green; Opal™520) at 1 : 200 ( 0.14 μg/ml) [Panel C] on human liver. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab195872 for 30 mins, ab275376 for 30 mins and ab150301 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Carboxypeptidase A (ab278044, magenta; Opal™690), anti-Cytokeratin 19 (ab195872, green; Opal™520) and anti-Insulin (ab181547, red; Opal™570) on human pancreas. Panel B : anti-Carboxypeptidase A stained on acinar cells. Panel C : anti-Cytokeratin 19 stained on centroacinar cells and ducts. Panel D : anti-Insulin stained on beta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab278044 at 1/4000 dilution (0.135 μg/ml), ab195872 at 1/8000 dilution (0.127 μg/ml), and ab181547 at 1/20000 dilution (0.053 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Human breast tissue labelling Fibrillin 1 with ab315384 at 1 : 100 dilution (B), Cytokeratin 19 with ab52625 at 1 : 6000 dilution (C) and Hormone sensitive lipase/HSL with ab322344 at 1 : 2000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Fibrillin 1 (green; Opal™570), anti-Cytokeratin 19 (magenta; Opal™690) and anti-Hormone sensitive lipase/HSL (gray; Opal™570) on human breast.

Panel B : anti-Fibrillin 1 staining stromal connective tissue in human breast.

Panel C : ant-Cytokeratin 19 staining epithelium in human breast.

Panel D : ant-Hormone sensitive lipase/HSL staining adipocytes in human breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315384, ab52625 and ab322344 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using the same antibody clone in a different buffer formulation (ab52625).

ab52625 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52625 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (Alexa Fluor® 647). Please refer to ab192980 for protocol details.

ab192980 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 100% methanol (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192980 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor^® 488 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Clone EP1580Y (ab195872) has been successfully conjugated by Abcam. This image was generated using Anti-Cytokeratin 19 antibody [EP1580Y] (PE). Please refer to ab224981 for protocol details.

Overlay histogram showing HeLa cells stained with ab224981 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224981, 1/1000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody.

Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Immunocytochemistry/Immunofluorescence analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labelling Cytokeratin 19 (green) with purified ab52625 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by ab150120 AlexaFluor^®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (ab150077).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Immunofluorescent staining of MCF-7 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab52625 at a dilution of 1/200. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200 and the cells were counter stained with DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^™ tissue staining C Peptide with ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse pancreas.
Panel B : anti-C Peptide staining beta cells in mouse pancreas.
Panel C : anti-Chymotrypsin staining exocrine gland in mouse pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab318203 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat liver.
Panel B : anti-COL4A2 staining endothelium in rat liver.
Panel C : anti-ALDH1L1 staining hepatocytes in rat liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^® tissue staining C Peptide with ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (gray; Opal^™570) on rat pancreas.
Panel B : anti-C Peptide staining beta cells in rat pancreas.
Panel C : anti-Chymotrypsin staining exocrine gland in rat pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab318203 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining C Peptide with ab314215 at a 1/500 dilution, ab278044 anti-Carboxypeptidase A used at 1/4000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Carboxypeptidase A (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat pancreas.
Panel B : anti-C Peptide staining beta cells in rat pancreas.
Panel C : anti-Carboxypeptidase A staining exocrine gland in rat pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab278044 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse liver.
Panel B : anti-COL4A2 staining endothelium in mouse liver.
Panel C : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

This data was developed using ab52625, the same antibody clone in a different buffer formulation. Different batches of ab52625 were tested on HepG2 (Human hepatocellular carcinoma epithelial cell) lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (<a href='/en-us/products/primary-antibodies/cytokeratin-19-antibody-ep1580y-cytoskeleton-marker-ab52625'>ab52625</a>)

Predicted band size: 44 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Unpurified ab52625 showing negative staining in Glioma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Unpurified ab52625 showing positive staining in Endometrial carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

Unpurified ab52625 showing positive staining in Gastric adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52625).

• OI-RD Scanning

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OI-RD Scanning - Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (AB195872)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.