Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung cancer.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on pancreatic ducts.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human spleen.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y(human neuroblastoma epithelial cell, Left) / MCF7(human breast adenocarcinoma epithelial cell, Right) cells labelling Cytokeration 19 with ab323561 at 1/1000 dilution (0.1 ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/5000 dilution was used as the secondary antibody.

Negative control : SH-SY5Y.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human thyroid cancer.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeration 19 with ab323561 at 1/4000 ( 0.2495 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in MCF7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : SH-SY5Y.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/200 10ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

• WB

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Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human stomach tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-fc-hrp-preadsorbed-ab97090'>ab97090</a>) at 1/20000 dilution

Observed band size: 44 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : SH-SY5Y (PMID : 30650643 and PMID : 27345400). Spleen (PMID : 24309898).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 4 seconds; Lanes 3-4 : 37 seconds

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-fc-hrp-preadsorbed-ab97090'>ab97090</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Spleen (PMID : 24309898)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution

Lane 1:

Mouse colon tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat colon tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-fc-hrp-preadsorbed-ab97090'>ab97090</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 26s