Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)

Rat Recombinant Monoclonal Cytokeratin 19 antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (AB323561), expandable thumbnail

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information Keratin, type I cytoskeletal 19

Function

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Alternative names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19

Rat Recombinant Monoclonal Cytokeratin 19 antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1580Y
Purification technique: Ion exchange chromatography
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This rat antibody has been engineered from a RabMAb parent antibody (Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y(human neuroblastoma epithelial cell, Left) / MCF7(human breast adenocarcinoma epithelial cell, Right) cells labelling Cytokeration 19 with ab323561 at 1/1000 dilution (0.1 ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/5000 dilution was used as the secondary antibody.
Negative control: SH-SY5Y.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeration 19 with ab323561 at 1/4000 ( 0.2495 ug/ml) dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in MCF7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control: SH-SY5Y.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/200 10ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human spleen.

The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum.

The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human thyroid cancer.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human lung cancer.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Cytokeration 19 with ab323561 at 1/5000 (0.2 ug/ml) dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on pancreatic ducts.
The section was incubated with ab323561 for 30 mins at room temperature, followed by anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution
Lane 1: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Human stomach tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution
Observed band size: 44 kDa
Exposure time: 26s
Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: SH-SY5Y (PMID:30650643 and PMID:?27345400). Spleen (PMID: 24309898).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 4 seconds; Lanes 3-4: 37 seconds
All lanes: Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
Lane 3: Human colon tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution
Observed band size: 44 kDa, 36 kDa
Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Spleen (PMID: 24309898)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) (ab323561) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Rat colon tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution
Observed band size: 44 kDa, 36 kDa
Exposure time: 26s