AB133496

Anti-Cytokeratin 19 antibody [EPNCIR127B]

Name: Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) | Abcam
Brand: Abcam Limited
SKU: AB133496
Price: 255 USD
Availability: InStock
Rating: 5 (7 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(7 Reviews)

(89 Publications)

Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) is a rabbit monoclonal antibody detecting Cytokeratin 19 in Western Blot, IHC-P. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

View Alternative Names

Krt1-19, Krt19, Cytokeratin-19, Keratin-19, CK-19, K19

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Immunohistochemical analysis of paraffin embedded Mouse colon tissue labelling Cytokeratin 19 using unpurified ab133496 antibody at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Immunohistochemical analysis of mouse orofacial tissue, staining Cytokeratin 19 with unpurified ab133496.

Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/500 in diluent) for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/2) was used as the secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling Cytokeratin 19 with purified ab133496 at 1 : 500 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

Unknown

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution

Lane 1:

Mouse kidney lysates at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 40 kDa

false

Unknown

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

F9 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 40 kDa

false

Carrier free

Anti-Cytokeratin 19 antibody [EPNCIR127B] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPNCIR127B]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EPNCIR127B]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EPNCIR127B]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EPNCIR127B]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EPNCIR127B]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR127B

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Cytokeratin 19 antibody [EPNCIR127B] (ab133496) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse samples.

What is the molecular weight of Cytokeratin 19?
Anti-Cytokeratin 19 [EPNCIR127B] (ab133496) specifically detects a band for Cytokeratin 19 (UniProt: P08727) at a molecular weight of 44kDa.

Trusted by the scientific community
Anti-Cytokeratin 19 [EPNCIR127B] (ab133496) was first used in a scientific publication in 2012 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPNCIR127B] also available for your convenience: ab133496, Carrier free - ab233174, Alexa Fluor® 488 - ab311036, Alexa Fluor® 647 - ab311162, Alexa Fluor® 594 - ab311812, Alexa Fluor® 568 - ab313095, Alexa Fluor® 555 - ab313288, Alexa Fluor® 750 - ab321048

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle (By similarity).

See full target information Krt19

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Publications (89)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113171 PubMed40792027

2025

exoCasMINI: A T5 exonuclease fused CRISPR-Cas12f system with enhanced gene editing efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitong Liao,Kechen Chen,Shufeng Ma,Xiaofeng Yang,Peihan Chen,Sijie Li,Mengrao Li,Xin Zhang,Ying Lin

Genesis (New York, N.Y. : 2000) 63:e70022 PubMed40631533

2025

Development of a Hepatocyte-Specific Temporal Genetic Mouse Model Using Albumin Promoter-Driven FlpER2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Jiale Wang,Yang Liu,Gordon He,Yasmin Jahan-Mihan,Yuxi Wang,Jie Hu,Audrey Li,Faheem Muhammad,Yan Bi,Baoan Ji

Frontiers in transplantation 4:1612523 PubMed40626170

2025

Hypomethylating therapy mitigates acute allograft rejection in a murine lung transplant model.

Applications

Unspecified application

Species

Unspecified reactive species

Kristine M Yarnoff,William N Daccarett-Bojanini,Andres F Villabona-Rueda,Manuel Sollmann,Franco R D'Alessio,Jeffrey M Dodd-O

Cell & bioscience 15:84 PubMed40533862

2025

Generation of a spontaneous murine HPV + oral cancer model with site-specific oncogene insertion using CRISPR-SONIC.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Tao,Jason Murray,Hsin-Fang Tu,Darrell Fan,Ya-Chea Tsai,Ming-Hung Hu,Annie A Wu,Deyin Xing,Chien-Fu Hung,T-C Wu

Nature communications 16:5001 PubMed40442121

2025

hpCasMINI: An engineered hypercompact CRISPR-Cas12f system with boosted gene editing activity.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Ma,Kaitong Liao,Kechen Chen,Tong Cheng,Xiaofeng Yang,Peihan Chen,Sijie Li,Mengrao Li,Xin Zhang,Yanqun Zhang,Tao Huang,Xiaobo Wang,Lanfeng Wang,Ying Lin,Zhili Rong

Stem cell research & therapy 16:161 PubMed40165226

2025

Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghao Liu,Heng Zhou,Pengcheng Pang,Ye Liu,Jieying Situ,Junnan Wang,Yan He,Qingsong Ye

Science advances 11:eadq4416 PubMed40073121

2025

Targeting the NPY/NPY1R signaling axis in mutant p53-dependent pancreatic cancer impairs metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia R Chambers,Supitchaya Watakul,Peter Schofield,Anna E Howell,Jessie Zhu,Alice M H Tran,Nadia Kuepper,Daniel A Reed,Kendelle J Murphy,Lily M Channon,Brooke A Pereira,Victoria M Tyma,Victoria Lee,Michael Trpceski,Jake Henry,Pauline Melenec,Lea Abdulkhalek,Max Nobis,Xanthe L Metcalf,Shona Ritchie,Antonia Cadell,Janett Stoehr,Astrid Magenau,Diego Chacon-Fajardo,Jessica L Chitty,Savannah O'Connell,Anaiis Zaratzian,Michael Tayao,Andrew Da Silva,Ruth J Lyons,Leonard D Goldstein,Ashleigh Dale,Alexander Rookyard,Angela Connolly,Ben Crossett,Yen T H Tran,Peter Kaltzis,Claire Vennin,Marija Dinevska,David R Croucher,Jaswinder Samra,Anubhav Mittal,Robert J Weatheritt,Andrew Philp,Gonzalo Del Monte-Nieto,Lei Zhang,Ronaldo F Enriquez,Thomas R Cox,Yan-Chuan C Shi,Mark Pinese,Nicola Waddell,Hao-Wen Sim,Tatyana Chtanova,Yingxiao Wang,Anthony M Joshua,Lorraine Chantrill,Thomas R Jeffry Evans,Anthony J Gill,Jennifer P Morton,Marina Pajic,Daniel Christ,Herbert Herzog,Paul Timpson,David Herrmann

Molecular cancer 24:25 PubMed39825392

2025

Intercellular TIMP-1-CD63 signaling directs the evolution of immune escape and metastasis in KRAS-mutated pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-An Wang,Ya-Chin Hou,Yi-Kai Hong,Yu-Jing Tai,Chieh Shen,Pei-Chi Hou,Jhao-Lin Fu,Cheng-Lin Wu,Siao Muk Cheng,Daw-Yang Hwang,Yung-Yeh Su,Yan-Shen Shan,Shaw-Jenq Tsai

EMBO reports 26:880-907 PubMed39747668

2025

RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wai-Ling Macrina Lam,Gisela Gabernet,Tanja Poth,Melanie Sator-Schmitt,Morgana Barroso Oquendo,Bettina Kast,Sabrina Lohr,Aurora de Ponti,Lena Weiß,Martin Schneider,Dominic Helm,Karin Müller-Decker,Peter Schirmacher,Mathias Heikenwälder,Ursula Klingmüller,Doris Schneller,Fabian Geisler,Sven Nahnsen,Peter Angel

Journal of experimental & clinical cancer research : CR 43:253 PubMed39243039

2024

HSF1 is a prognostic determinant and therapeutic target in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cigliano,Isabella Gigante,Marina Serra,Gianpaolo Vidili,Maria M Simile,Sara Steinmann,Francesco Urigo,Eleonora Cossu,Giovanni M Pes,Maria P Dore,Silvia Ribback,Egle P Milia,Elena Pizzuto,Serena Mancarella,Li Che,Rosa M Pascale,Gianluigi Giannelli,Matthias Evert,Xin Chen,Diego F Calvisi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 19 antibody [EPNCIR127B]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

Western blot - Anti-Cytokeratin 19 antibody [EPNCIR127B] (AB133496)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Appropriate long-term storage conditions

Aliquoting information

Storage information

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Publications (89)

Product promise