Skip to main content

Mouse Monoclonal Cytokeratin 19 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT19.


Images

Immunocytochemistry - Anti-Cytokeratin 19 antibody [KRT19/800] (AB220193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (AB220193), expandable thumbnail
  • Western blot - Anti-Cytokeratin 19 antibody [KRT19/800] (AB220193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (AB220193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (AB220193), expandable thumbnail

Publications

  • Cell reports 29:1832-1847.e82019
    Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Ross Dobie,John R Wilson-Kanamori,Beth E P Henderson,James R Smith,Kylie P Matchett,Jordan R Portman,Karolina Wallenborg,Simone Picelli,Anna Zagorska,Swetha V Pendem,Thomas E Hudson,Minnie M Wu,Grant R Budas,David G Breckenridge,Ewen M Harrison,Damian J Mole,Stephen J Wigmore,Prakash Ramachandran,Chris P Ponting,Sarah A Teichmann,John C Marioni,Neil C Henderson
    PubMed 31722201

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human KRT19. Database link P08727

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-P
Human
Tested
Tested
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1-2 µg/mL

Notes

Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Species

Human

Dilution info

1-2 µg/mL

Notes

Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Associated Products

Select an associated product type

1 products for Alternative Version

    7 products for Alternative Product

      Target data

      Function

      Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

      Alternative names

      Cytokeratin-19, Keratin-19, CK-19, K19, KRT19

      Recommended products

      1. Loading...
      2. Loading...
      3. Loading...
      4. Loading...

      Mouse Monoclonal Cytokeratin 19 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT19.

      Alternative names

      Cytokeratin-19, Keratin-19, CK-19, K19, KRT19

      Key facts

      Isotype

      IgG1

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • Recombinant Full Length Protein corresponding to Human KRT19. Database link P08727
      Clone number

      KRT19/800

      Purification technique

      Affinity purification Protein A/G

      Light chain type

      kappa

      Concentration
      Loading...
      Purification notes

      ab220193 was purified from Bioreactor Concentrate by Protein A/G.

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Supplementary info

      Activity summary

      Cytokeratin 19 (CK19) also known as cytokera­tine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

      Biological function summary

      CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

      Pathways

      CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

      Associated diseases and disorders

      CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      9 product images

      • Immunocytochemistry - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunocytochemistry - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunocytochemistry analysis of MeOH fixed MCF7 (human breast adenocarcinoma cell line) cells labeling CK19 using ab220193 followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat colon tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Western blot - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Western blot - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        All lanes: Western blot - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        All lanes: Human lung lysate

        Predicted band size: 44 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human pancreas tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lung tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human bladder carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrial carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [KRT19/800] (ab220193)

        Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Cytokeratin 19 with ab220193 at 1 μg/ml dilution.

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com