AB9221

Anti-Cytokeratin 19 antibody [RCK108]

Name: Anti-Cytokeratin 19 antibody [RCK108] (ab9221) | Abcam
Brand: Abcam Limited
SKU: AB9221
Price: 470 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(28 Publications)

Anti-Cytokeratin 19 antibody [RCK108] (ab9221) is a mouse monoclonal antibody detecting Cytokeratin 19 in Flow Cytometry, IHC-P, IHC-Fr. Suitable for Human, Zebrafish.

- Over 10 publications
- Trusted since 2002

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Indirect immunoperoxidase staining of human small intestine paraffin tissue section with ab9221. Dilution 1 : 50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Indirect immunoperoxidase staining of human placenta paraffin tissue section with ab9221. Dilution 1 : 50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry on frozen tissue sections of human colon with ab9221 (1 : 500).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

ab9221 staining Cytokeratin 19 in human skin (sweat glands).
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section with ab9221. Dilution 1 : 50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry on a frozen tissue sections of human kidney with ab9221 (1 : 500).

Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Overlay histogram showing MCF-7 cells stained with ab9221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9221, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry on frozen section of human liver bile duct epithelium

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

ab9221 staining cytokeratin 19 in three-day-old Zebrafish tissue by Immunohistochemistry (Frozen sections).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RCK108

Isotype

IgG1

Carrier free

Reacts with

Human, Zebrafish

Applications

Flow Cyt, IHC-Fr, IHC-P

applications

Specificity

This antibody reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. It does not react with hepatocytes and hepatocellular carcinoma.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Cytokeratin 19 antibody [RCK108] (ab9221) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Zebrafish samples.

Trusted by the scientific community
Anti-Cytokeratin 19 [RCK108] (ab9221) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists
Anti-Cytokeratin 19 [RCK108] (ab9221) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1593226 PubMed40395933

2025

Generation and functional characterization of tuft cells in non-human primate pancreatic ducts through organoid culture systems.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Sakaguchi,Chiemi Kimura-Nakajima,Akihiko Inaba,Yoshiko Hatano,Hanako Ogawa,Yuichi Koshiishi,Keisuke Tanaka,Tatsuya Kometani,Makoto Ohmoto,Koji Sato,Hiroo Imai,Ken Iwatsuki

Cell reports. Medicine 5:101692 PubMed39163864

2024

CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Tang,Quan Zheng,Qi Wang,Yaru Zhao,Preeta Ananthanarayanan,Chiara Reina,Berina Šabanović,Ke Jiang,Ming-Hsin Yang,Clara Csilla Meny,Huimin Wang,Mette Ø Agerbaek,Thomas Mandel Clausen,Tobias Gustavsson,Chenlei Wen,Felice Borghi,Alfredo Mellano,Elisabetta Fenocchio,Vanesa Gregorc,Anna Sapino,Thor G Theander,Da Fu,Alexandra Aicher,Ali Salanti,Baiyong Shen,Christopher Heeschen

Nature communications 15:5266 PubMed38902237

2024

In vivo CRISPR screens reveal SCAF1 and USP15 as drivers of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastien Martinez,Shifei Wu,Michael Geuenich,Ahmad Malik,Ramona Weber,Tristan Woo,Amy Zhang,Gun Ho Jang,Dzana Dervovic,Khalid N Al-Zahrani,Ricky Tsai,Nassima Fodil,Philippe Gros,Steven Gallinger,G Gregory Neely,Faiyaz Notta,Ataman Sendoel,Kieran Campbell,Ulrich Elling,Daniel Schramek

Nature communications 15:5117 PubMed38879551

2024

Chip collection of hepatocellular carcinoma based on O heterogeneity from patient tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sewoom Baek,Hyun-Su Ha,Jeong Su Park,Min Jeong Cho,Hye-Seon Kim,Seung Eun Yu,Seyong Chung,Chansik Kim,Jueun Kim,Ji Youn Lee,Yerin Lee,Hyunjae Kim,Yujin Nam,Sungwoo Cho,Kyubae Lee,Ja Kyung Yoon,Jin Sub Choi,Dai Hoon Han,Hak-Joon Sung

Journal of cell science 137: PubMed37676312

2023

Trophoblast organoids with physiological polarity model placental structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Yang,Pengfei Liang,Huanghe Yang,Carolyn B Coyne

iScience 26:107675 PubMed37680467

2023

Integrative analysis of single-cell RNA-seq and ATAC-seq reveals heterogeneity of induced pluripotent stem cell-derived hepatic organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Hwan Kim,Seon Ju Mun,Jeong-Hwan Kim,Myung Jin Son,Seon-Young Kim

Cell metabolism 35:1209-1226.e13 PubMed37172577

2023

Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Wang,So Yeon Kim,Wei Tu,Jieun Kim,Alexander Xu,Yoon Mee Yang,Michitaka Matsuda,Lien Reolizo,Takashi Tsuchiya,Sandrine Billet,Alexandra Gangi,Mazen Noureddin,Ben A Falk,Sungjin Kim,Wei Fan,Mourad Tighiouart,Sungyong You,Michael S Lewis,Stephen J Pandol,Dolores Di Vizio,Akil Merchant,Edwin M Posadas,Neil A Bhowmick,Shelly C Lu,Ekihiro Seki

Advanced healthcare materials 12:e2202422 PubMed37086259

2023

An Automation Workflow for High-Throughput Manufacturing and Analysis of Scaffold-Supported 3D Tissue Arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Ruonan Cao,Nancy T Li,Simon Latour,Jose L Cadavid,Cassidy M Tan,Ari Forman,Hartland W Jackson,Alison P McGuigan

JOR spine 6:e1238 PubMed36994456

2023

Harmonization and standardization of nucleus pulposus cell extraction and culture methods.

Applications

Unspecified application

Species

Unspecified reactive species

Shaghayegh Basatvat,Frances C Bach,Marcos N Barcellona,Abbie L Binch,Conor T Buckley,Brian Bueno,Nadeen O Chahine,Ana Chee,Laura B Creemers,Stefan Dudli,Bailey Fearing,Stephen J Ferguson,Jennifer Gansau,Benjamin Gantenbein,Rahul Gawri,Juliane D Glaeser,Sibylle Grad,Julien Guerrero,Lisbet Haglund,Paula A Hernandez,Judith A Hoyland,Charles Huang,James C Iatridis,Svenja Illien-Junger,Liufang Jing,Petra Kraus,Lisanne T Laagland,Gernot Lang,Victor Leung,Zhen Li,Thomas Lufkin,Josette C van Maanen,Emily E McDonnell,Chris J Panebianco,Steven M Presciutti,Sanjna Rao,Stephen M Richardson,Sarah Romereim,Tara C Schmitz,Jordy Schol,Lori Setton,Dmitriy Sheyn,Joseph W Snuggs,Y Sun,Xiaohong Tan,Marianna A Tryfonidou,Nam Vo,Dong Wang,Brandon Williams,Rebecca Williams,S Tim Yoon,Christine L Le Maitre

eLife 11: PubMed35975985

2022

Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Yang,Eleanor C Semmes,Cristian Ovies,Christina Megli,Sallie Permar,Jennifer B Gilner,Carolyn B Coyne

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 19 antibody [RCK108]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (AB9221)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

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