Mouse Monoclonal Cytokeratin 19 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 19 protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [SPM561] - BSA and Azide free (AB212569), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-19-antibody-spm561-bsa-and-azide-free-ab212569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131445.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [SPM561] - BSA and Azide free (AB212569)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Keratin, type I cytoskeletal 19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08727
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Alternative names
Cytokeratin-19, Keratin-19, CK-19, K19, KRT19
Recommended products
Mouse Monoclonal Cytokeratin 19 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 19 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Keratin, type I cytoskeletal 19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08727
- Carrier free
- Yes
- Clone number
- SPM561
- Purification technique
- Affinity purification Protein A/G
- Specificity
ab212569 reacts with the rod domain of Human Cytokeratin 19.
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
- Biological function summary
CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.
- Pathways
CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.
- Associated diseases and disorders
CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [SPM561] - BSA and Azide free (ab212569), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-19-antibody-spm561-bsa-and-azide-free-ab212569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131445.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [SPM561] - BSA and Azide free (ab212569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [SPM561] - BSA and Azide free (ab212569)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labeling Cytokeratin 19 with ab212569 at 1 μg/mL.
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