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AB236751

Anti-Cytokeratin 2 antibody

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Rabbit Polyclonal Cytokeratin 2P antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT76 aa 150-300.

View Alternative Names

KRT2B, KRT2P, KRT76, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 2 antibody (AB236751)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 2 antibody (AB236751)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Cytokeratin 2 (green) with ab236751 at 1/133 dilution in ICC/IF. Counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2 antibody (AB236751)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2 antibody (AB236751)

Paraffin-embedded human endometrial cancer tissue stained for Cytokeratin 2 using ab236751 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot - Anti-Cytokeratin 2 antibody (AB236751)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 2 antibody (AB236751)

All lanes:

Western blot - Anti-Cytokeratin 2 antibody (ab236751) at 1/500 dilution

All lanes:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human KRT76 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q01546

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 2 also known as KRT2 is a type II cytokeratin with a molecular weight of approximately 66 kDa. This protein is primarily expressed in the suprabasal layers of the epidermis especially in the upper spinous and granular layers of the skin. KRT2 plays a role in forming and maintaining the structural integrity of the epithelial cells due to its ability to assemble into intermediate filaments that provide mechanical support and resilience to epithelial tissues.
Biological function summary

Cytokeratin 2 contributes to epidermal differentiation and the formation of the skin barrier. It acts as part of the cytoskeletal network in keratinocytes providing structural support to the cells. Cytokeratin 2 is often found operating together with other keratin proteins as part of a complex. Its filamentous structure helps resist stress and external forces allowing the skin to withstand physical and chemical insults.

Pathways

Cytokeratin 2 has a significant role in keratinocyte differentiation and the skin barrier formation pathways. It is intricately related to other members of the keratin family such as Cytokeratin 1 (KRT1) which further regulate these processes. Cytokeratin 2 expression affects pathways that control cell proliferation and apoptosis within the skin ensuring the balance between cell division and cell death necessary for maintaining healthy skin tissue.

Deficiencies or mutations in Cytokeratin 2 can lead to Ichthyosis Bullosa of Siemens a condition characterized by blistering and peeling skin. Mutations may affect the stability and assembly of keratin filaments compromising skin integrity. Cytokeratin 2 abnormalities connect with other cytokeratin proteins like KRT1 which also contribute to similar skin disorders. Understanding its role and interactions with other proteins may assist in the development of treatments for keratin-associated skin diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably contributes to terminal cornification.
See full target information KRT76
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