AB53120

Anti-Cytokeratin 20 antibody

Name: Anti-Cytokeratin 20 antibody (ab53120) | Abcam
Brand: Abcam Limited
SKU: AB53120

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(8 Publications)

Rabbit Polyclonal Cytokeratin 20 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human KRT20.

View Alternative Names

Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (AB53120)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (AB53120)

ab53120 at 1/50 dilution staining Cytokeratin 20 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-Cytokeratin 20 antibody (AB53120)

All lanes:

Western blot - Anti-Cytokeratin 20 antibody (ab53120) at 1/300 dilution

Lane 1:

HeLa cell extract

Lane 2:

HeLa cell extract with immunising peptide

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KRT20. The exact immunogen used to generate this antibody is proprietary information.

P35900

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.

Biological function summary

Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.

Pathways

Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.

Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

See full target information KRT20

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6032 PubMed40595652

2025

EZH2 loss promotes gastric squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengsha Zhang,Ailing Zhong,Hongyu Liu,Lei Zhao,Yingjie Wang,Zhenghao Lu,Lanxin Zhang,Xiangyu Pan,Zuoyu Liang,Limin Gao,Xuelan Chen,Jian Wang,Siqi Dai,Xudong Wan,Manli Wang,Jingyao Chen,Jiajia Du,Shuang Chen,Tingfa Peng,Jingyang Cai,Liuxiang Chen,Yang Yang,Chaoxin Xiao,Menglin Yao,Jing Xu,Runkuan Qin,Chan Yang,Baohong Wu,Qi Zhang,Shengzhuo Liu,Chengjian Zhao,Hongxin Deng,Yuan Wang,Lu Chen,Jiankun Hu,Bing Hu,Shengyong Yang,Feifei Na,Linjie Guo,Yu Liu,Chong Chen

Molecular biology of the cell 35:ar139 PubMed39356795

2024

The rat bladder umbrella cell keratin network: Organization, dependence on the plectin cytolinker, and responses to bladder filling.

Applications

Unspecified application

Species

Unspecified reactive species

Wily G Ruiz,Dennis R Clayton,Tanmay Parakala-Jain,Marianela G Dalghi,Jonathan Franks,Gerard Apodaca

American journal of physiology. Renal physiology 323:F299-F321 PubMed35834272

2022

Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA interstitial cells are fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis R Clayton,Wily G Ruiz,Marianela G Dalghi,Nicolas Montalbetti,Marcelo D Carattino,Gerard Apodaca

JCI insight 6: PubMed34464353

2021

Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception.

Applications

Unspecified application

Species

Unspecified reactive species

Marianela G Dalghi,Wily G Ruiz,Dennis R Clayton,Nicolas Montalbetti,Stephanie L Daugherty,Jonathan M Beckel,Marcelo D Carattino,Gerard Apodaca

Toxicologic pathology 46:488-510 PubMed29966501

2018

Immunohistochemistry in Investigative and Toxicologic Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Kyathanahalli S Janardhan,Heather Jensen,Natasha P Clayton,Ronald A Herbert

Molecular cancer research : MCR 16:1568-1578 PubMed29934328

2018

BMP7 Signaling in -Deficient Stromal Cells Provokes Epithelial Carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hans Petter Eikesdal,Lisa M Becker,Yingqi Teng,Akane Kizu,Julienne L Carstens,Keizo Kanasaki,Hikaru Sugimoto,Valerie S LeBleu,Raghu Kalluri

American journal of physiology. Renal physiology 313:F85-F102 PubMed28331065

2017

Urothelial proliferation and regeneration after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

F Aura Kullmann,Dennis R Clayton,Wily G Ruiz,Amanda Wolf-Johnston,Christian Gauthier,Anthony Kanai,Lori A Birder,Gerard Apodaca

Toxicologic pathology 42:555-64 PubMed23703846

2013

Mutation spectra of Kras and Tp53 in urethral and lung neoplasms in B6C3F1 mice treated with 3,3',4,4'-tetrachloroazobenzene.

Applications

Unspecified application

Species

Mouse

Sachin Bhusari,David E Malarkey,Hue-Hua Hong,Yu Wang,Tiwanda Masinde,Michael Nolan,Michelle J Hooth,Isabel A Lea,Daphne Vasconcelos,Robert C Sills,Mark J Hoenerhoff

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cytokeratin 20 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (AB53120)

Western blot - Anti-Cytokeratin 20 antibody (AB53120)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise