AB314073

Anti-Cytokeratin 20 antibody [BLR105H] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 20 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KRT20 aa 250-300.

View Alternative Names

Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20

4 Images

Supplier Data

Immunocytochemistry - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Detection of human Cytokeratin 20 in FFPE HT-29 cells by ICC Antibody : Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody [BLR105H]. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Detection of human Cytokeratin 20 in FFPE colon carcinoma by IHC Antibody : Rabbit anti-Cytokeratin 20 recombinant monoclonal antibody [BLR105H] (A700-105). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HT-29 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiCytokeratin-20 recombinant monoclonal antibody [BLR105H] (A700-105) used for IP at 20 µl/mg lysate. Cytokeratin-20 was also immunoprecipitated by rabbit anti-Cytokeratin-20 antibody BL1464B-2D4. For blotting immunoprecipitated Cytokeratin-20, A700-105 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

false

Supplier Data

Western blot - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Samples : Whole cell lysate (50 µg) from HEK293T, HeLa, Jurkat, MOLT-4, HT-29, Hep-G2, HDLM-2, and MDAMB-231 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Cytokeratin-20 recombinant monoclonal antibody [BLR105H] (A700-105) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 1 second. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (ab314073) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

Jurkat Whole cell lysate at 50 µg

Lane 4:

MOLT-4 Whole cell lysate at 50 µg

Lane 5:

HT-29 Whole cell lysate at 50 µg

Lane 6:

Hep-G2 Whole cell lysate at 50 µg

Lane 7:

HDLM-2 Whole cell lysate at 50 µg

Lane 8:

MDA-MB-231 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR105H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KRT20 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P35900

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.

Biological function summary

Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.

Pathways

Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.

Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

See full target information KRT20

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 20 antibody [BLR105H] - BSA free

Immunocytochemistry - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Immunoprecipitation - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Western blot - Anti-Cytokeratin 20 antibody [BLR105H] - BSA free (AB314073)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise