Rabbit Polyclonal Cytokeratin 20 antibody. Cytoskeleton marker. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 50-300.
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- Cell death & disease 14:3182023Single-cell sequencing reveals homogeneity and heterogeneity of the cytopathological mechanisms in different etiology-induced AKI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Chen,Yinshuang Li,Ying Yuan,Kunmei Lai,Keng Ye,Yujiao Lin,Ruilong Lan,Hong Chen,Yanfang XuPubMed 37169762
- Communications biology 5:13262022An integrated map of fibroblastic populations in human colon mucosa and cancer tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiying Li,Ran Lu,Linjuan Shu,Yulin Chen,Jin Zhao,Junlong Dai,Qiaorong Huang,Xue Li,Wentong Meng,Feiwu Long,Yuan Li,Chuanwen Fan,Zongguang Zhou,Xianming MoPubMed 36463319
- Nature genetics 54:1827-18382022The NALCN channel regulates metastasis and nonmalignant cell dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric P Rahrmann,David Shorthouse,Amir Jassim,Linda P Hu,Mariaestela Ortiz,Betania Mahler-Araujo,Peter Vogel,Marta Paez-Ribes,Atefeh Fatemi,Gregory J Hannon,Radhika Iyer,Jay A Blundon,Filipe C Lourenço,Jonathan Kay,Rosalynn M Nazarian,Benjamin A Hall,Stanislav S Zakharenko,Douglas J Winton,Liqin Zhu,Richard J GilbertsonPubMed 36175792
- American journal of clinical and experimental urology 9:416-4342021Patient derived models of bladder cancer enrich the signal of the tumor cell transcriptome facilitating the analysis of the tumor cell compartment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichalis Mastri,Swathi Ramakrishnan,Shruti D Shah,Ellen Karasik,Bryan M Gillard,Michael T Moser,Bailey K Farmer,Gissou Azabdaftari,Gurkamal S Chatta,Anna Woloszynska,Kevin H Eng,Barbara A Foster,Wendy J HussPubMed 34993263
- Gastroenterology 157:1599-1614.e22019Mouse Models of Human Gastric Cancer Subtypes With Stomach-Specific CreERT2-Mediated Pathway Alterations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTherese Seidlitz et. al.PubMed 31585123
- Oncology letters 12:4505-45092016Small bowel metastasis from pancreatic cancer in a long-term survival patient with synchronous advanced malignant pleural mesothelioma: A case report and literature review.
- Cell death & disease 4:e8572013Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KRT20 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P35900
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes - |
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Alternative names
Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20
Recommended products
Rabbit Polyclonal Cytokeratin 20 antibody. Cytoskeleton marker. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 50-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KRT20 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link P35900
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
- Biological function summary
Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.
- Pathways
Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.
- Associated diseases and disorders
Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
ab97511 staining Cytokeratin 20 in Mouse urinary bladder by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Sample was incubated with primary antibody at a 1:500 dilution.
Western blot - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
10% SDS PAGE
All lanes: Western blot - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511) at 1/3000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
ab97511 staining Cytokeratin 20 in HT29 xenograft by Immunohistochemistry (IHC-P - paraformaldehyde - fixed, paraffin embedded sections). Samples were incubated with primary antibody at 1:100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
Immunofluorescence analysis of Cytokeratin 20 in paraformaldehyde fixed HeLa, using ab97511 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody - Cytoskeleton Marker (ab97511)
Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using ab97511 at 1/100 dilution.
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