Rabbit Recombinant Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Images
![Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--western-blot-img165857.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22611.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunocytochemistry-immunofluorescence-img42067.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589)")
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--flow-cytometry-intracellular-img115307.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130852.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (AB219589)")
Publications
Filter by
- Nutrients 8:2016Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness.
- Pflugers Archiv : European journal of physiology 468:1171-11812016The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.
- The American journal of pathology 182:449-592012Deficiency in metabolic regulators PPARγ and PTEN cooperates to drive keratinizing squamous metaplasia in novel models of human tissue regeneration.Applications:WBReactive species:Unspecified reactive speciesDouglas W Strand,David J DeGraff,Ming Jiang,Mansoureh Sameni,Omar E Franco,Harold D Love,William J Hayward,Opal Lin-Tsai,Anne Y Wang,Justin M M Cates,Bonnie F Sloane,Robert J Matusik,Simon W HaywardPubMed 23219716
- American journal of physiology. Endocrinology and 303:E253-642012The effects of 13 wk of liraglutide treatment on endocrine and exocrine pancreas in male and female ZDF rats: a quantitative and qualitative analysis revealing no evidence of drug-induced pancreatitis.
- Proceedings of the National Academy of Sciences of 109:2078-832012Three differentiation states risk-stratify bladder cancer into distinct subtypes.
- British journal of cancer 106:133-402011Specific activity of cyclin-dependent kinase I is a new potential predictor of tumour recurrence in stage II colon cancer.Applications:ICC/IFReactive species:HumanE C M Zeestraten,M Maak,M Shibayama,T Schuster,U Nitsche,T Matsushima,S Nakayama,K Gohda,H Friess,C J H van de Velde,H Ishihara,R Rosenberg,P J K Kuppen,K-P JanssenPubMed 22108518
- Cancer research 71:3246-562011mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways.Applications:IHC-PReactive species:HumanPat Gulhati,Kanika A Bowen,Jianyu Liu,Payton D Stevens,Piotr G Rychahou,Min Chen,Eun Y Lee,Heidi L Weiss,Kathleen L O'Connor,Tianyan Gao,B Mark EversPubMed 21430067
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Common marmoset | Predicted | Predicted | Predicted | Predicted |
| Goat | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Goat, Pig, Common marmoset | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Goat, Pig, Common marmoset | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Goat, Pig, Common marmoset | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Goat, Pig, Common marmoset | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
4 products for Alternative Version
Target data
Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Alternative names
Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20
Recommended products
Rabbit Recombinant Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1622Y
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen of this antibody is 73% homolog with Mouse-Cytokeratin 20. This antibody gives positive results for mouse samples in Western Blot only. Therefore we do not recommend this antibody for mouse samples and do not cover mouse with our Abpromise guarantee.
- Dissociation constant
- 3.1 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab219589 is the carrier-free version of Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
- Biological function summary
Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.
- Pathways
Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.
- Associated diseases and disorders
Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--western-blot-img165857.jpg "Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
False colour image of Western blot: Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 was shown to bind specifically to Cytokeratin 20. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
All lanes: Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126) at 1/10000 dilution
Lane 1: HT-29 cell lysate at 20 µg
Lane 2: DLD-1 cell lysate at 20 µg
Lane 3: SW480 cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
Lane 5: MCF7 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 45 kDa, 45-50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22611.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 at 1/100 dilution staining Cytokeratin 20 in human colon adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunocytochemistry-immunofluorescence-img42067.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Cytokeratin 20 with purified Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 at 1/500. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--flow-cytometry-intracellular-img115307.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Flow Cytometry (Intracellular) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Overlay histogram showing LoVo (Human colorectal adenocarcinoma cell line) cells stained with Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130852.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 showing positive staining in human colonic adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133528.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 showing positive staining in human urinary bladder transitional carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135331.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 showing negative staining in human hepatocellular carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58207.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126 showing negative staining in human breast carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133271.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
Fluorescent immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126. Green-CK20 red-PI
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker ab76126).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--oi-rd-scanning-img83227.jpg "OI-RD Scanning - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
OI-RD Scanning - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133528.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135331.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58207.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133271.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
![OI-RD Scanning - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-epr1622y-bsa-and-azide-free-ab219589--oi-rd-scanning-img83227.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Cytokeratin 20 antibody [EPR1622Y] - BSA and Azide free (ab219589)")
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