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AB237881

Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 150-350.

View Alternative Names

Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)

Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.

Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
  • WB

Unknown

Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)

All lanes:

Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] (<a href='/en-us/products/primary-antibodies/cytokeratin-20-antibody-krt20-1992-ab238034'>ab238034</a>) at 2 µg/mL

All lanes:

HT-29 (human colorectal adenocarcinoma cell line) cell lysate

Predicted band size: 48 kDa

false

Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
  • Protein Array

Unknown

Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)

This data was produced with ab238034, the same antibody in a different formulation with BSA and Azide.
ab238034 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KRT20/1992

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT20 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P35900

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
Biological function summary

Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.

Pathways

Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.

Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
See full target information KRT20

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:3120-3140.e29 PubMed38714197

2024

Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Shovik Bandyopadhyay,Michael P Duffy,Kyung Jin Ahn,Jonathan H Sussman,Minxing Pang,David Smith,Gwendolyn Duncan,Iris Zhang,Jeffrey Huang,Yulieh Lin,Barbara Xiong,Tamjid Imtiaz,Chia-Hui Chen,Anusha Thadi,Changya Chen,Jason Xu,Melissa Reichart,Zachary Martinez,Caroline Diorio,Chider Chen,Vinodh Pillai,Oraine Snaith,Derek Oldridge,Siddharth Bhattacharyya,Ivan Maillard,Martin Carroll,Charles Nelson,Ling Qin,Kai Tan
View all publications

Product promise

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