Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free
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(1 Publication)
Mouse Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 150-350.
View Alternative Names
Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.
- WB
Unknown
Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
All lanes:
Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] (<a href='/en-us/products/primary-antibodies/cytokeratin-20-antibody-krt20-1992-ab238034'>ab238034</a>) at 2 µg/mL
All lanes:
HT-29 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 48 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)
This data was produced with ab238034, the same antibody in a different formulation with BSA and Azide.
ab238034 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Reactivity data
Properties and storage information
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Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.
Pathways
Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.
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Target data
Publications (1)
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Cell 187:3120-3140.e29 PubMed38714197
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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