Mouse Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 150-350.
Images
![Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--protein-array-img2009.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)")
![Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--western-blot-img839.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106012.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115153.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (AB237881)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human KRT20 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P35900
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Incubate with primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Alternative names
Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20
Recommended products
Mouse Monoclonal Cytokeratin 20 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KRT20 aa 150-350.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human KRT20 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P35900
- Carrier free
- Yes
- Clone number
- KRT20/1992
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
- Biological function summary
Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.
- Pathways
Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.
- Associated diseases and disorders
Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
![Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--protein-array-img2009.jpg "Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)")
Protein Array - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)
This data was produced with Anti-Cytokeratin 20 antibody [KRT20/1992] ab238034, the same antibody in a different formulation with BSA and Azide.
Anti-Cytokeratin 20 antibody [KRT20/1992] ab238034 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--western-blot-img839.jpg "Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)")
Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)
All lanes: Western blot - Anti-Cytokeratin 20 antibody [KRT20/1992] (Anti-Cytokeratin 20 antibody [KRT20/1992] ab238034) at 2 µg/mL
All lanes: HT-29 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106012.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-20-antibody-krt20-1992-bsa-and-azide-free-ab237881--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115153.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [KRT20/1992] - BSA and Azide free (ab237881)
Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 20 using ab237881 at 2 μg/ml in immunohistochemical analysis.
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