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AB64090

Anti-Cytokeratin 20 antibody [SP33]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

5

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 20 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Rat samples. Cited in 6 publications.

View Alternative Names

Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Cytokeratin 20 with ab64090 at 1/100 dilution (17.9 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64090 for 30 mins at room temperature.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Immunocytochemistry/ Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Cytokeratin 20 with purified ab64090 at 1/500 (3.58 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 20 with ab64090 at a 1/100 dilution in immunohistochemical analysis.

Flow Cytometry - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Flow cytometry analysis of HT-29 (human colorectal adenocarcinoma) labeling Cytokeratin 20 with purified ab64090 at 1/1700 dilution (1.05 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Cytokeratin 20 with ab64090 at 1/100 dilution (17.9 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64090 for 30 mins at room temperature.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 20 antibody [SP33] (AB64090)

Immunohistochemistry (Frozen) analysis of rat colon tissue section labeling Cytokeratin 20 with purified ab64090 at 1/1000 (1.8 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Carrier free

    Anti-Cytokeratin 20 antibody [SP33] - BSA and Azide free

  • Unconjugated

    Anti-Cytokeratin 20 antibody [SP33], prediluted

  • 660 APC

    APC Anti-Cytokeratin 20 antibody [SP33]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [SP33]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Cytokeratin 20 antibody [SP33]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [SP33]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cytokeratin 20 antibody [SP33]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Cytokeratin 20 antibody [SP33]

  • 578 PE

    PE Anti-Cytokeratin 20 antibody [SP33]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP33

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-Fr, IHC-P, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1700", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary antibody for 30 minutes at room temperature.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary antibody for 30 minutes at room temperature.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
Biological function summary

Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.

Pathways

Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.

Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
See full target information KRT20
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:257 PubMed40213090

2025

Robot‑assisted laparoscopic partial cystectomy for urachal carcinoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Chujin Ye,Qifan Xie,Zhiye Liu,Fangqianyu Zhong,Kun Wei,Xiaoyong Pu

Cell reports. Medicine 5:101851 PubMed39657667

2024

Multi-modal analysis reveals tumor and immune features distinguishing EBV-positive and EBV-negative post-transplant lymphoproliferative disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Toh,Andrea J Reitsma,Tetsuya Tajima,Sheren F Younes,Chimere Ezeiruaku,Kayla C Jenkins,Josselyn K Peña,Shuchun Zhao,Xi Wang,Esmond Y Z Lee,Marla C Glass,Laurynas Kalesinskas,Ananthakrishnan Ganesan,Irene Liang,Joy A Pai,James T Harden,Francesco Vallania,Edward A Vizcarra,Govind Bhagat,Fiona E Craig,Steven H Swerdlow,Julie Morscio,Daan Dierickx,Thomas Tousseyn,Ansuman T Satpathy,Sheri M Krams,Yasodha Natkunam,Purvesh Khatri,Olivia M Martinez

Biomedical reports 20:61 PubMed38476609

2024

Metastatic gallbladder cancer presenting as numb chin syndrome: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Murahashi,Edward Hosea Ntege,Kentaro Ide,Nobuyuki Maruyama,Jumpei Shirakawa,Hiroki Koyama,Toshihiro Kawano,Takahiro Goto,Yusuke Shimizu,Kazuhide Nishihara,Hiroyuki Nakamura

Journal of gastroenterology and hepatology 37:898-907 PubMed35244298

2022

Modeling colorectal cancer: A bio-resource of 50 patient-derived organoid lines.

Applications

Unspecified application

Species

Unspecified reactive species

Rebekah M Engel,Thierry Jardé,Karen Oliva,Genevieve Kerr,Wing Hei Chan,Sara Hlavca,David Nickless,Stuart K Archer,Raymond Yap,Pravin Ranchod,Stephen Bell,Ann Niap,Christine Koulis,Ashley Chong,Simon Wilkins,Trevor C Dale,Andrew J Hollins,Paul J McMurrick,Helen E Abud

Journal of microscopy and ultrastructure 11:34-40 PubMed37144164

2021

Pioglitazone Induces Dysplastic Urothelial Changes in Urinary Bladder of Experimental Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A M Abdel-Hamid,Alaa El-Din L Firgany

International journal of experimental pathology 100:369-377 PubMed32040227

2020

Dysplastic urothelial changes accompany empagliflozin administration in urinary bladder of experimental diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A M Abdel-Hamid,Alaa El-Din L Firgany
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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