AB64091

Anti-Cytokeratin 20 antibody [SP33], prediluted

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 20 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cytokeratin-20, Keratin-20, Protein IT, CK-20, K20, KRT20

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33], prediluted (AB64091)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33], prediluted (AB64091)

Immunohistochemical analysis of paraffin-embedded tissue labelling Cytokeratin 20 with ab64091, prediluted, followed by a secondary antibody. Cytoplasmic staining is observed. Counter stained with Hematoxylin.

Carrier free

Anti-Cytokeratin 20 antibody [SP33] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 20 antibody [SP33]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 20 antibody [SP33]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cytokeratin 20 antibody [SP33]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 20 antibody [SP33]
578 PE

PE Anti-Cytokeratin 20 antibody [SP33]
660 APC

APC Anti-Cytokeratin 20 antibody [SP33]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Cytokeratin 20 antibody [SP33]
Unconjugated

Anti-Cytokeratin 20 antibody [SP33]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP33

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1", "IHCP-species-notes": "<p>Ready-to-use for 30 min at RT.</p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: Tris buffered saline, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.

Biological function summary

Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.

Pathways

Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.

Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

See full target information KRT20

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:257 PubMed40213090

2025

Robot‑assisted laparoscopic partial cystectomy for urachal carcinoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Chujin Ye,Qifan Xie,Zhiye Liu,Fangqianyu Zhong,Kun Wei,Xiaoyong Pu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 20 antibody [SP33], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [SP33], prediluted (AB64091)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise