AB77869

Anti-Cytokeratin 3/CK-3 antibody [AE5]

Name: Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab77869) | Abcam
Brand: Abcam Limited
SKU: AB77869
Rating: 3 (1 reviews)

(1 Review)

(17 Publications)

Mouse Monoclonal K2C3 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 17 publications.

View Alternative Names

65 kDa cytokeratin, Cytokeratin-3, Keratin-3, Type-II keratin Kb3, CK-3, K3, KRT3

1 Images

Flow Cytometry (Intracellular) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (AB77869)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (AB77869)

Overlay histogram showing A431 cells stained with ab77869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77869, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Carrier free

Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free
Unconjugated

Anti-Cytokeratin 3/CK-3 antibody [AE5]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg for 10^6 Cells", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 3 also known as CK-3 or CK3 is a type II cytokeratin protein with a molecular weight of approximately 64 kDa. This protein often coexists with Cytokeratin 12 and together they form the epithelial keratins. CK-3 is expressed predominantly in corneal epithelial cells. The presence of CK-3 in these cells helps maintain structural integrity and resilience against mechanical stress through its network of intermediate filaments. Various forms like CK-3 IgG and IGG CK3 have been studied to understand the unique and important role of cytokeratins in epithelial tissues.

Biological function summary

CK-3 plays an important role in the structural framework inside corneal epithelial cells. It participates in forming a complex of intermediate filaments providing necessary strength and stability to the cells. Such a framework is important for protecting the epithelium under physical stressors while also supporting the dynamic processes of cell proliferation and differentiation. These cytokeratin complexes contribute to the cellular process by supporting the resilience of epithelial cells.

Pathways

The involvement of CK-3 in cytoskeletal organization highlights its connection to certain critical signaling pathways such as the epithelial-to-mesenchymal transition (EMT) and cell differentiation pathways. The interaction of CK-3 with proteins like Cytokeratin 12 regulates these pathways and contributes to maintaining corneal epithelial homeostasis and integrity. Such interactions enable CK-3 to help maintain the proper functioning and regeneration of epithelial barriers.

CK-3's alteration or dysfunction may contribute to corneal-related diseases such as keratitis and corneal dystrophies. Abnormalities or mutations in CK-3 affect corneal integrity leading to compromised vision and disease symptoms. In these disorders its interaction with proteins like AE5 which participates in corneal structural pathways can be disrupted leading to pathophysiological conditions affecting the corneal epithelium. Understanding CK-3's role in these diseases may help guide therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT3

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3604 PubMed37330515

2023

Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Yao,Xiaoyi Mo,Shanshan Liu,Qian Wang,Maowen Xie,Wenhao Lou,Shiyan Chen,Taisong Pan,Ke Chen,Dezhong Yao,Yuan Lin

Frontiers in bioengineering and biotechnology 10:883977 PubMed35769102

2022

Electron-Beam Irradiated Recombinant Human Collagen-Phosphorylcholine Corneal Implants Retain Pro-Regeneration Capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona C Simpson,Mohammed Mirazul Islam,Oleksiy Buznyk,Elle Edin,Marc Groleau,Monika Kozak-Ljunggren,Federica M Magrelli,Dina B AbuSamra,Pablo Argüeso,James Chodosh,Aneta Liszka,Per Fagerholm,May Griffith

Investigative ophthalmology & visual science 63:31 PubMed35212722

2022

Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chen Sun,Kai-Feng Hung,Tzu-Yun Li,Yu-An Chang,Po-Ting Yeh,Fung-Rong Hu

Journal of ophthalmology 2021:2406646 PubMed34976406

2021

Topical Vitamin C Promotes the Recovery of Corneal Alkali Burns in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Zufeng Chen,Lin Liu,Xiaoyun Ma,Jun Zou

Scientific reports 10:16671 PubMed33028837

2020

In situ-forming collagen hydrogel crosslinked via multi-functional PEG as a matrix therapy for corneal defects.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Maria Fernandes-Cunha,Karen Mei Chen,Fang Chen,Peter Le,Ju Hee Han,Leela Ann Mahajan,Hyun Jong Lee,Kyung Sun Na,David Myung

Royal Society open science 6:191796 PubMed31903218

2019

Influence of polydimethylsiloxane substrate stiffness on corneal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia Masterton,Mark Ahearne

Scientific reports 9:18088 PubMed31792300

2019

Simple oral mucosal epithelial transplantation in a rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Inamochi,Akiko Tomioka,Kohdai Kitamoto,Takashi Miyai,Tomohiko Usui,Makoto Aihara,Satoru Yamagami

Investigative ophthalmology & visual science 60:3854-3862 PubMed31529118

2019

The Role of the miR-21/SPRY2 Axis in Modulating Proangiogenic Factors, Epithelial Phenotypes, and Wound Healing in Corneal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Fukang Yuan,Lin Liu,Zufeng Chen,Xiaoyun Ma,Zhen Lin,Jun Zou

Experimental eye research 185:107681 PubMed31150636

2019

Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Zheng,Chenglei Tian,Tingjun Fan,Bin Xu

BioMed research international 2018:7627329 PubMed30519584

2018

Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux.

Applications

Unspecified application

Species

Unspecified reactive species

Shivapriya Shivakumar,Trailokyanath Panigrahi,Rohit Shetty,Murali Subramani,Arkasubhra Ghosh,Nallathambi Jeyabalan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 3/CK-3 antibody [AE5]

Flow Cytometry (Intracellular) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (AB77869)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise