Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (AB255962)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab68260).

Immunohistochemical analysis of paraffin-embedded human squamous papilloma of cornea tissue labeling Cytokeratin 3/CK-3 with ab68260 at 1/500 dilution, followed by ready to use secondary. Positive staining on human squamous papilloma of cornea tissue is observed. The section was incubated with ab68260 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (AB255962)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab68260).

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cell line labeling Cytokeratin 3/CK-3 with ab68260 at 1/500 dilution (red) compared with a Mouse IgG, monoclonal Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG ab150113 (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

• WB

Lab

Western blot - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (AB255962)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab68260). Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control.

All lanes:

Western blot - Anti-Cytokeratin 3/CK-3 antibody [AE5] (<a href='/en-us/products/primary-antibodies/cytokeratin-3-ck-3-antibody-ae5-ab68260'>ab68260</a>) at 1/5000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector containing a His tag®, whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with Cytokeratin full length expression vector containing a His tag®, whole cell lysate at 15 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 64 kDa

Observed band size: 65 kDa

false

Exposure time: 3s