Rabbit Recombinant Monoclonal K2C4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97646.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunocytochemistry-immunofluorescence-img125166.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316)")
![Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--western-blot-img142073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316)")
![Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunoprecipitation-img10044.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316)")
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--flow-cytometry-intracellular-img2505.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (AB247316)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Version
Alternative names
Cytokeratin-4, Keratin-4, Type-II keratin Kb4, CK-4, K4, CYK4, KRT4
Recommended products
Rabbit Recombinant Monoclonal K2C4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Cytokeratin-4, Keratin-4, Type-II keratin Kb4, CK-4, K4, CYK4, KRT4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP1599Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247316 is the carrier-free version of Anti-Cytokeratin 4 antibody [EP1599Y] ab51599.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97646.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
This data was developed using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunocytochemistry-immunofluorescence-img125166.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
This data was developed using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599, the same antibody clone in a different buffer formulation.ICC/IF image of Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--western-blot-img142073.jpg "Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)")
Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
This data was developed using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] (Anti-Cytokeratin 4 antibody [EP1599Y] ab51599) at 1/1000 dilution
All lanes: A431 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 59 kDa
![Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--immunoprecipitation-img10044.jpg "Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)")
Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
This data was developed using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599, the same antibody clone in a different buffer formulation.
Purified Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 at 1/50 dilution (2μg) immunoprecipitating Cytokeratin 4 in HaCaT whole cell lysate.
Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10μg
Lane 2 (+): Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 + HaCaT whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 in HaCaT whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 59 kDaAll lanes: Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] (Anti-Cytokeratin 4 antibody [EP1599Y] ab51599)
Predicted band size: 57 kDa
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-4-antibody-ep1599y-bsa-and-azide-free-ab247316--flow-cytometry-intracellular-img2505.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)")
Flow Cytometry (Intracellular) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
This data was developed using Anti-Cytokeratin 4 antibody [EP1599Y] ab51599, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 4 with purified Anti-Cytokeratin 4 antibody [EP1599Y] ab51599 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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