Anti-Cytokeratin 5 + 6 + 18 antibody [LP34]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085μg/ml, followed by a ready to use secondary. Positive staining on human bladder carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085μg/ml, followed by a ready to use secondary. Positive staining on human pancreatic carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 5 + 6 + 18 with ab40 at 1/1000 dilution, followed by a ready to use secondary. Positive staining on human skin is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085μg/ml, followed by a ready to use secondary. Positive staining on the epithelial cells of human tonsil tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Lab

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 secs; Lane 2-3 : 3 secs.

All lanes:

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 45 kDa

false

• WB

Lab

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (AB40)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 1/1000 dilution

All lanes:

Human skin lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Observed band size: 58 kDa

false

Exposure time: 114s