Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) is a rabbit polyclonal antibody that is used to detect Cytokeratin 5 in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Over 80 publications
- Trusted since 2007
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- Immunity, inflammation and disease 11:e11042024Pro-inflammatory cytokine IL-6 regulates LMO4 expression in psoriatic keratinocytes via AKT/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Tu,Wei Wei,Qiantong Xiang,Wenwen Wang,Siping Zhang,Haisheng ZhouPubMed 38156380
- iScience 26:1069252023Targeting the PRC2-dependent epigenetic program alleviates urinary tract infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunming Guo,Mingyi Zhao,Xinbing Sui,Zarine Balsara,Songhui Zhai,Michael Ahdoot,Yingsheng Zhang,Christa M Lam,Ping Zhu,Xue LiPubMed 37332606
- Molecular metabolism 71:1017062023Inceptor correlates with markers of prostate cancer progression and modulates insulin/IGF1 signaling and cancer cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Wissmiller,Sara Bilekova,Andras Franko,Stefan Z Lutz,Miriam Katsburg,Sebastian Gulde,Natalia S Pellegata,Arnulf Stenzl,Martin Heni,Lucia Berti,Hans-Ulrich Häring,Heiko LickertPubMed 36931467
- The American journal of pathology 193:680-6892023Lung Remodeling Regions in Long-Term Coronavirus Disease 2019 Feature Basal Epithelial Cell Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKangyun Wu,Yong Zhang,Stephen R Austin,Huiqing Yin-Declue,Derek E Byers,Erika C Crouch,Michael J HoltzmanPubMed 36868468
- Advanced biology 7:e22001602023DNA Methylation Dynamics During Esophageal Epithelial Regeneration Following Repair with Acellular Silk Fibroin Grafts in Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren A Urban,Jiachun Li,Gokhan Gundogdu,Annie Trinh,Hanjuan Shao,Travis Nguyen,Joshua R Mauney,Timothy L DowningPubMed 36658732
- Cancer research 83:264-2842022JAK-STAT Signaling in Inflammatory Breast Cancer Enables Chemotherapy-Resistant Cell States.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura E Stevens,Guillermo Peluffo,Xintao Qiu,Daniel Temko,Anne Fassl,Zheqi Li,Anne Trinh,Marco Seehawer,Bojana Jovanović,Maša Alečković,Callahan M Wilde,Renee C Geck,Shaokun Shu,Natalie L Kingston,Nicholas W Harper,Vanessa Almendro,Alanna L Pyke,Shawn B Egri,Malvina Papanastasiou,Kendell Clement,Ningxuan Zhou,Sarah Walker,Jacqueline Salas,So Yeon Park,David A Frank,Alexander Meissner,Jacob D Jaffe,Piotr Sicinski,Alex Toker,Franziska Michor,Henry W Long,Beth A Overmoyer,Kornelia PolyakPubMed 36409824
- International journal of molecular sciences 23:2022Immunohistochemical Changes in the Testicular Excurrent Duct System of Healthy, Male Japanese Quail () Observed at 4, 6-7, 12, and 52 Weeks of Age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed I A Ibrahim,June H Williams,Christo J BothaPubMed 36430504
- eLife 11:2022Contribution of -labeled cells to alveolar regeneration is independent of tuft cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuachao Huang,Yinshan Fang,Ming Jiang,Yihan Zhang,Jana Biermann,Johannes C Melms,Jennifer A Danielsson,Ying Yang,Li Qiang,Jia Liu,Yiwu Zhou,Manli Wang,Zhihong Hu,Timothy C Wang,Anjali Saqi,Jie Sun,Ichiro Matsumoto,Wellington V Cardoso,Charles W Emala,Jian Zhu,Benjamin Izar,Hongmei Mou,Jianwen QuePubMed 36129169
- Cytokine 160:1560222022Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria M Szwarc,Lan Hai,Vineet K Maurya,Kimal Rajapakshe,Dimuthu Perera,Michael M Ittmann,Qianxing Mo,Yong Lin,Matthew L Bettini,Cristian Coarfa,John P LydonPubMed 36099756
- STAR protocols 3:1013902022Primary culture of tracheal aspirate-derived human airway basal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaurang M Amonkar,Richard Wagner,Kamakshi Bankoti,Jessica E Shui,Xingbin Ai,Paul H LerouPubMed 35600918
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Keratin, type II cytoskeletal 5. The exact immunogen used to generate this antibody is proprietary information. Database link P13647
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Alternative names
58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5
Recommended products
Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) is a rabbit polyclonal antibody that is used to detect Cytokeratin 5 in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Over 80 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Keratin, type II cytoskeletal 5. The exact immunogen used to generate this antibody is proprietary information. Database link P13647
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab53121 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Cytokeratin 5?
Anti-Cytokeratin 5 - Cytoskeleton Marker (ab53121) specifically detects a band for Cytokeratin 5 (UniProt: P13647) at a molecular weight of 62kDa.
Trusted by the scientific community
Anti-Cytokeratin 5 - Cytoskeleton Marker (ab53121) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-Cytokeratin 5 - Cytoskeleton Marker (ab53121) has over 5 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)
ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Western blot - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)
All lanes: Western blot - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) at 1/300 dilution
Lane 1: HepG2 cell extract
Lane 2: HepG2 cell extract with immunising peptide
Predicted band size: 62 kDa
Observed band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121)
ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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