Anti-Cytokeratin 5 antibody - Cytoskeleton Marker

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (AB53121)

ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (AB53121)

ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

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Western blot - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (AB53121)

All lanes:

Western blot - Anti-Cytokeratin 5 antibody - Cytoskeleton Marker (ab53121) at 1/300 dilution

Lane 1:

HepG2 cell extract

Lane 2:

HepG2 cell extract with immunising peptide

Predicted band size: 62 kDa

Observed band size: 62 kDa

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