Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker is a rabbit recombinant monoclonal antibody that is used to detect Cytokeratin 5 in Flow cytometry (Intra), ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 160 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1601Y is the most widely used clone for Cytokeratin 5 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48361.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img56962.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41958.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)")
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img60019.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)")
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img164580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)")
Publications
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- Methods in molecular biology (Clifton, N.J.) 2736:53-642024Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRika Yasuhara,Seya Kang,Rino Tokumasu,Kenji MishimaPubMed 36749482
- British journal of cancer 130:369-3792023Urine-derived bladder cancer organoids (urinoids) as a tool for cancer longitudinal response monitoring and therapy adaptation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBastiaan J Viergever,Daniëlle A E Raats,Veerle Geurts,Jasper Mullenders,Trudy N Jonges,Michiel S van der Heijden,Johan H van Es,Onno Kranenburg,Richard P MeijerPubMed 38102228
- BMC urology 23:1692023Distinct effects of Fgf7 and Fgf10 on the terminal differentiation of murine bladder urothelium revealed using an organoid culture system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuto Suda,Yuka Matsumoto,Takanori Ochi,Hiroyuki Koga,Nobutaka Hattori,Atsuyuki Yamataka,Tetsuya NakamuraPubMed 37875848
- Cell death & disease 14:6752023Kindlin-2 in myoepithelium controls luminal progenitor commitment to alveoli in mouse mammary gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenbin Wang,Lei Zhang,Bing Li,Jiagui Song,Miao Yu,Jing Zhang,Ceshi Chen,Jun Zhan,Hongquan ZhangPubMed 37833248
- Nature communications 14:59952023Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang,Chenyu Chu,Li Liu,Chenbing Wang,Chen Hu,Shengan Rung,Yi Man,Yili QuPubMed 37752124
- Nature communications 14:56652023MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZinab O Doha,Xiaoyan Wang,Nicholas L Calistri,Jennifer Eng,Colin J Daniel,Luke Ternes,Eun Na Kim,Carl Pelz,Michael Munks,Courtney Betts,Sunjong Kwon,Elmar Bucher,Xi Li,Trent Waugh,Zuzana Tatarova,Dylan Blumberg,Aaron Ko,Nell Kirchberger,Jennifer A Pietenpol,Melinda E Sanders,Ellen M Langer,Mu-Shui Dai,Gordon Mills,Koei Chin,Young Hwan Chang,Lisa M Coussens,Joe W Gray,Laura M Heiser,Rosalie C SearsPubMed 37704631
- Cell death discovery 9:3392023YAP is required for prostate development, regeneration, and prostate stem cell function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Xie,Linpei Guo,Qianwang Ma,Wenyi Zhang,Zhao Yang,Zhun Wang,Shuanghe Peng,Keruo Wang,Simeng Wen,Zhiqun Shang,Yuanjie NiuPubMed 37689711
- Diagnostic pathology 18:982023Heterotopia of salivary gland tissue in the pancreas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandrina Martens,Katarina Coolens,Catharina Olsen,Pierre Lefesvre,Ilse RoomanPubMed 37649044
- iScience 26:1075682023Estrogen receptor alpha regulates uterine epithelial lineage specification and homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason A Rizo,Kimberly M Davenport,Wipawee Winuthayanon,Thomas E Spencer,Andrew M KelleherPubMed 37622003
- Cancers 15:2023Α Humanized RANKL Transgenic Mouse Model of Progestin-Induced Mammary Carcinogenesis for Evaluation of Novel Therapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthi Kolokotroni,Evi Gkikopoulou,Vagelis Rinotas,Lydia Ntari,Danae Zareifi,Maritina Rouchota,Sophia Sarpaki,Ilias Lymperopoulos,Leonidas G Alexopoulos,George Loudos,Maria C Denis,Niki Karagianni,Eleni DouniPubMed 37568820
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Tested | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Alternative names
58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5
Recommended products
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker is a rabbit recombinant monoclonal antibody that is used to detect Cytokeratin 5 in Flow cytometry (Intra), ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 160 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Antibody clone EP1601Y is the most widely used clone for Cytokeratin 5 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1601Y
- Purification technique
- Affinity purification Protein A
- Specificity
Mouse reactivity is based on IHC (positive tissues: Liver, lung, brain and skin). However, WB was negative for Mouse brain, heart, kidney and spleen. There is background staining in mouse and rat islet.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) was first used in a scientific publication in 1977 and has been cited over 163 times in peer reviewed journals. It's performance in IHC in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) has 10 independent reviews from customers.
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) specifically detects Cytokeratin 5 (UniProt ID: P13647; Molecular weight: 63kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1601Y - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free ab214586.
Antibody clone EP1601Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, APC, PE (Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] ab193894, Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] ab193895, HRP Anti-Cytokeratin 5 antibody [EP1601Y] ab193896, APC Anti-Cytokeratin 5 antibody [EP1601Y] ab224984, PE Anti-Cytokeratin 5 antibody [EP1601Y] ab224985).
Cytokeratin 5 (CK5) is a key diagnostic marker used to identify and classify certain cancers, such as basal-like breast carcinomas and squamous cell carcinomas. Its expression is linked to high-grade tumors and poorer prognosis in various cancers, including urothelial and colorectal cancers.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
20 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48361.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing positive staining in squamous cell cervical carcinoma tissue.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img56962.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Image from Khanom R. et al PLoS One. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163. eCollection 2016.Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Colocalization of KRT5, KRT6 and KRT17 in HSC3 cellsImmunocytochemistry in HSC3 (human oral squamous carcinoma cell line) cells. Scale bar, 10 μm.
(Taken from Figure S3 of Khanom et al)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41958.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img60019.jpg "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
The lysates were prepared in 1%SDS Hot lysis method.
Observed MW: 62kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution
Lane 1: Human skin lysates prepared in RIPA lysis method at 20 µg
Lane 2: Human skin lysates prepared in 1%SDS Hot lysis method at 20 µg
Lane 3: Mouse skin lysates prepared in RIPA lysis method at 20 µg
Lane 4: Mouse skin lysates prepared in 1%SDS Hot lysis method at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 62 kDa
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img164580.jpg "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Different batches of ab52635 were tested on Rat skin lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 62 kDa.
All lanes: Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Predicted band size: 62 kDa
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img20788.jpg "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Blocking and diluting buffer: 5% NFDM/TBST.
The lysates were prepared in 1%SDS Hot lysis method.
All lanes: Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution
Lane 1: Human fetal skin lysates at 20 µg
Lane 2: Rat skin lysates at 20 µg
Lane 3: Mouse skin lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img58722.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104701.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--flow-cytometry-intracellular-img27452.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94852.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img87893.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
A431 cells stained with unpurified ab52635 at 1/100 - 1/250
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img31255.jpg "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
All lanes: Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution
All lanes: A431 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135500.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img52686.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43967.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132476.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Unpurified ab52635 showing positive staining in basal cell breast carcinoma tissue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416680.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
IHC image of Cytokeratin 5 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab52635, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img416079.jpg "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
Blocking buffer: 5% NFDM /TBST.
All lanes: Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution
Lane 1: N-GST tagged full-length recombinant human Cytokeratin 6A protein at 10 ng
Lane 2: N-GST tagged full-length recombinant human Cytokeratin 5 protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 87 kDa
Exposure time: 10s
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img20788.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img58722.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--flow-cytometry-intracellular-img27452.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94852.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunocytochemistry-immunofluorescence-img87893.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img31255.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135500.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img52686.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43967.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132476.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416680.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
![Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-ep1601y-cytoskeleton-marker-ab52635--western-blot-img416079.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)")
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