Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing positive staining in squamous cell cervical carcinoma tissue.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr^®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

A431 cells stained with unpurified ab52635 at 1/100 - 1/250

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

IHC image of Cytokeratin 5 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab52635, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Colocalization of KRT5, KRT6 and KRT17 in HSC3 cells

Immunocytochemistry in HSC3 (human oral squamous carcinoma cell line) cells. Scale bar, 10 μm.

(Taken from Figure S3 of Khanom et al)

Image from Khanom R. et al PLoS One. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163. eCollection 2016.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1 : 200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1 : 200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1 : 200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Blocking buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution

Lane 1:

N-GST tagged full-length recombinant human Cytokeratin 6A protein at 10 ng

Lane 2:

N-GST tagged full-length recombinant human Cytokeratin 5 protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 87 kDa

false

Exposure time: 10s

• WB

Lab

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Blocking and diluting buffer : 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method.

All lanes:

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution

Lane 1:

Human fetal skin lysates at 20 µg

Lane 2:

Rat skin lysates at 20 µg

Lane 3:

Mouse skin lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

• WB

Unknown

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

The lysates were prepared in 1%SDS Hot lysis method.

Observed MW : 62kDa

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution

Lane 1:

Human skin lysates prepared in RIPA lysis method at 20 µg

Lane 2:

Human skin lysates prepared in 1%SDS Hot lysis method at 20 µg

Lane 3:

Mouse skin lysates prepared in RIPA lysis method at 20 µg

Lane 4:

Mouse skin lysates prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

false

• WB

Lab

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Different batches of ab52635 were tested on Rat skin lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 62 kDa.

All lanes:

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Predicted band size: 62 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing positive staining in basal cell breast carcinoma tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.

• WB

Unknown

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

All lanes:

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false