AB259429

Anti-Cytokeratin 5 antibody [KRT5/3594]

Name: Anti-Cytokeratin 5 antibody [KRT5/3594] (ab259429) | Abcam
Brand: Abcam Limited
SKU: AB259429
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal Cytokeratin 5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus.

View Alternative Names

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] (AB259429)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] (AB259429)

Formalin-fixed, paraffin-embeded human cervical carcinoma tissue labeling Cytokeratin 5 using ab259429 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] (AB259429)

Formalin-fixed, paraffin-embeded human pancreas tissue labeling Cytokeratin 5 using ab259429 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] (AB259429)

Formalin-fixed, paraffin-embeded human prostate carcinoma tissue labeling Cytokeratin 5 using ab259429 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] (AB259429)

Formalin-fixed, paraffin-embeded human prostate carcinoma tissue labeling Cytokeratin 5 using ab259429 at 2μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KRT5/3594

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13647

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

See full target information Keratin, type II cytoskeletal 5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38786035

2024

Long-Term Maintenance of Viable Human Endometrial Epithelial Cells to Analyze Estrogen and Progestin Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Assad Riaz,Franziska Louisa Kary,Alexandra Jensen,Felix Zeppernick,Ivo Meinhold-Heerlein,Lutz Konrad

Frontiers in medicine 9:841170 PubMed35252273

2022

Immune Cells Profiles in the Different Sites of COVID-19-Affected Lung Lobes in a Single Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Sadahiro Iwabuchi,Kyohei Miyamoto,Mayuko Hatai,Yurina Mikasa,Masahiro Katsuda,Shin-Ichi Murata,Toshikazu Kondo,Hiroki Yamaue,Shinichi Hashimoto

View all publications

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