Mouse Monoclonal Cytokeratin 5 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135534.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49216.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118524.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124082.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (AB259432)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13647
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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6 products for Alternative Product
Target data
Function
Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
Alternative names
58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5
Recommended products
Mouse Monoclonal Cytokeratin 5 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13647
- Carrier free
- Yes
- Clone number
- KRT5/3594
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab259432 is the carrier-free version of Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135534.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)
Formalin-fixed, paraffin-embeded human cervical carcinoma tissue labeling Cytokeratin 5 using Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429 at 2μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49216.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)
Formalin-fixed, paraffin-embeded human prostate carcinoma tissue labeling Cytokeratin 5 using Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429 at 2μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118524.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)
Formalin-fixed, paraffin-embeded human prostate carcinoma tissue labeling Cytokeratin 5 using Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429 at 2μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432), expandable thumbnail](https://content.abcam.com/products/images/cytokeratin-5-antibody-krt5-3594-bsa-and-azide-free-ab259432--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124082.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [KRT5/3594] - BSA and Azide free (ab259432)
Formalin-fixed, paraffin-embeded human pancreas tissue labeling Cytokeratin 5 using Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429 at 2μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Cytokeratin 5 antibody [KRT5/3594] ab259429).
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