Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free

13 product images

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  Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling Cytokeratin 5 with Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).
  

  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer
  tissue sections labeling Cytokeratin 5 with Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution (2.46 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human prostate cancer, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-Cytokeratin 5 antibody [SP27] ab64081 for 30 mins at room temperature. This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 5 with purified Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/30 (8.2 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  

  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 (2.5 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  

  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 5 with purified Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/30 (8.2 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  

  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling Cytokeratin 5 with Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution (2.46 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on rat skin, performed on a Leica Biosystems BOND™ RX instrument.
  

  The section was incubated with Anti-Cytokeratin 5 antibody [SP27] ab64081 for 30 mins at room temperature.

  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Cytokeratin 5 with Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution (2.46 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse skin, performed on a Leica Biosystems BOND™ RX instrument
  The section was incubated with Anti-Cytokeratin 5 antibody [SP27] ab64081 for 30 mins at room temperature. This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung squamous cell cancer tissue sections labeling Cytokeratin 5 with Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution (2.46 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on tumor cells of human lung squamous cell cancer, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-Cytokeratin 5 antibody [SP27] ab64081 for 30 mins at room temperature. This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 5 using Anti-Cytokeratin 5 antibody [SP27] ab64081 at 1/100 dilution in immunohistochemical analysis.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 5 antibody [SP27] ab64081).

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  Multiplex immunohistochemistry - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Prostate Specific Antigen (Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113, red; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Nkx3.1 (Anti-Nkx3.1 antibody [EPR16653] ab196020, gray; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on cytoplasm of luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-p63 stained on nucleus of luminal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113 (1/2000), ab236216 (1/400) and Anti-Nkx3.1 antibody [EPR16653] ab196020 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-p63 (Anti-p63 antibody [EPR5701] ab124762, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on cytoplasm of basal cells. Panel D: anti-p63 stained on nucleus of basal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-p63 antibody [EPR5701] ab124762 (1/5000), ab236216 (1/400), and Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Multiplex immunohistochemistry - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Collagen VI (Anti-Collagen VI antibody [EPR17072] - BSA and Azide free ab271938, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-Collagen VI stained on stroma. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Collagen VI antibody [EPR17072] - BSA and Azide free ab271938 (1/500), ab236216 (1/400), and Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
  This image was generated using Anti-Cytokeratin 5 antibody [SP27] ab64081, the same clone, but with a different buffer formulation.

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  Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free (ab236216)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytokeratin 5 antibody [SP27] ab64081).
  Flow cytometry overlay histogram showing left A-431 positive cells and right negative MCF7 stained with Anti-Cytokeratin 5 antibody [SP27] ab64081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (Anti-Cytokeratin 5 antibody [SP27] ab64081) (1x 106 in 100μl at 1.0μg/ml (1/2080)) for 30min at 22°C.
  
  The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
  
  Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
  
  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.