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AB17130

Anti-Cytokeratin 5 antibody [XM26]

4

(4 Reviews)

|

(20 Publications)

Mouse Monoclonal Cytokeratin 5 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5.

View Alternative Names

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [XM26] (AB17130)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [XM26] (AB17130)

Formalin-fixed, paraffin-embeded human tonsil tissue stained for Cytokeratin 5 using ab17130 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.

Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (AB17130)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (AB17130)

Overlay histogram showing A431 cells stained with ab17130 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17130, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

XM26

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Keratin, type II cytoskeletal 5. The exact immunogen used to generate this antibody is proprietary information.

P13647

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/75", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
See full target information Keratin, type II cytoskeletal 5
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:140 PubMed40102996

2025

Airway basal stem cell-derived extracellular vesicles modulate proliferation, migration and collagen deposition of fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Lisi Luo,Huijie Yang,Junfeng Huang,Difei Chen,Yushan He,Jinsheng Lin,Haikang Zeng,Chu Hua,Zikai Lin,Minting Wu,Yuqin Ma,Qilin Deng,Ming Liu,Shiyue Li

Scientific reports 14:6774 PubMed38514727

2024

Mechanosensitive TRPV4 channel guides maturation and organization of the bilayered mammary epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Kärki Tytti,Koskimäki Sanna,Guenther Carla,Pirhonen Jonatan,Rajakylä Kaisa,Tojkander Sari

Stem cells translational medicine 12:838-848 PubMed37804518

2023

Autologous Airway Basal Cell Transplantation Alleviates Airway Epithelium Defect in Recurrent Benign Tracheal Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Shun Ye,Di-Fei Chen,Ming Liu,Yu-Long Luo,Huan-Jie Chen,Hai-Kang Zeng,Jing-Wei Liu,Yi-Ping Zhu,Xin-Yu Song,Chang-Qin Lin,Zhu-Quan Su,Shi-Yue Li

Developmental cell 58:2428-2446.e9 PubMed37652013

2023

Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Ragazzini,Stefan Boeing,Luca Zanieri,Mary Green,Giuseppe D'Agostino,Kerol Bartolovic,Ana Agua-Doce,Maria Greco,Sara A Watson,Antoniana Batsivari,Linda Ariza-McNaughton,Asllan Gjinovci,David Scoville,Andy Nam,Adrian C Hayday,Dominique Bonnet,Paola Bonfanti

eLife 11: PubMed36129169

2022

Contribution of -labeled cells to alveolar regeneration is independent of tuft cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huachao Huang,Yinshan Fang,Ming Jiang,Yihan Zhang,Jana Biermann,Johannes C Melms,Jennifer A Danielsson,Ying Yang,Li Qiang,Jia Liu,Yiwu Zhou,Manli Wang,Zhihong Hu,Timothy C Wang,Anjali Saqi,Jie Sun,Ichiro Matsumoto,Wellington V Cardoso,Charles W Emala,Jian Zhu,Benjamin Izar,Hongmei Mou,Jianwen Que

Life science alliance 5: PubMed35922154

2022

Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Gimano D Amatngalim,Lisa W Rodenburg,Bente L Aalbers,Henriette Hm Raeven,Ellen M Aarts,Dounia Sarhane,Sacha Spelier,Juliet W Lefferts,Iris Al Silva,Wilco Nijenhuis,Sacha Vrendenbarg,Evelien Kruisselbrink,Jesse E Brunsveld,Cornelis M van Drunen,Sabine Michel,Karin M de Winter-de Groot,Harry G Heijerman,Lukas C Kapitein,Magarida D Amaral,Cornelis K van der Ent,Jeffrey M Beekman

Experimental animals : PubMed33473097

2021

Establishment and histopathological study of patient-derived xenograft models and primary cell lines of epithelioid malignant peritoneal mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Ran Yang,Zhi-Gao Chen,Zhong-He Ji,Yu-Lin Lin,Jue Zhang,Ru Ma,Zhao Li,Xi Jiang,Qian Chen,Xue-Mei DU,Yan Li

Scientific reports 10:14683 PubMed32895467

2020

TRPV6 calcium channel directs homeostasis of the mammary epithelial sheets and controls epithelial mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Tytti Kärki,Eeva Kaisa Rajakylä,Anna Acheva,Sari Tojkander

Matrix biology : journal of the International Soci 83:48-59 PubMed31302245

2019

Biallelic KRT5 mutations in autosomal recessive epidermolysis bullosa simplex, including a complete human keratin 5 "knock-out".

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Vahidnezhad,Leila Youssefian,Maryam Daneshpazhooh,Hamidreza Mahmoudi,Ariana Kariminejad,Judith Fischer,Julie Christiansen,Holm Schneider,Alyson Guy,Lu Liu,John A McGrath,Cristina Has,Jouni Uitto

Genome medicine 11:8 PubMed30777124

2019

BAP1 haploinsufficiency predicts a distinct immunogenic class of malignant peritoneal mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Raunak Shrestha,Noushin Nabavi,Yen-Yi Lin,Fan Mo,Shawn Anderson,Stanislav Volik,Hans H Adomat,Dong Lin,Hui Xue,Xin Dong,Robert Shukin,Robert H Bell,Brian McConeghy,Anne Haegert,Sonal Brahmbhatt,Estelle Li,Htoo Zarni Oo,Antonio Hurtado-Coll,Ladan Fazli,Joshua Zhou,Yarrow McConnell,Andrea McCart,Andrew Lowy,Gregg B Morin,Tianhui Chen,Mads Daugaard,S Cenk Sahinalp,Faraz Hach,Stephane Le Bihan,Martin E Gleave,Yuzhuo Wang,Andrew Churg,Colin C Collins
View all publications
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