AB18586

Anti-Cytokeratin 6 antibody [Ks6.KA12]

Name: Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586) | Abcam
Brand: Abcam Limited
SKU: AB18586
Rating: 5 (4 reviews)

(4 Reviews)

(17 Publications)

Mouse Monoclonal K2C6C antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT6C.

View Alternative Names

KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C

1 Images

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (AB18586)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (AB18586)

Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2μg/1x10⁶ cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1]ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Ks6.KA12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KRT6C.

P48668

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified by protein A affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information KRT6C

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 65:8 PubMed38315493

2024

Urban Particulate Matter Triggers Meibomian Gland Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqian Tu,Ren Liu,Jianwen Xue,Bing Xiao,Jing Li,Lingyi Liang

Skin health and disease 3:e245 PubMed37799359

2023

Inhibition of vascular endothelial growth factor-A downregulates angiogenesis in psoriasis: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Luengas-Martinez,Dina Ismail,Ralf Paus,Helen S Young

International journal of molecular sciences 24: PubMed37047109

2023

Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon Seo

Biomedicines 11: PubMed36672635

2023

New Data on the Features of Skin Barrier in Hidradenitis Suppurativa.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Somogyi,Zsolt Dajnoki,Lilla Szabó,Krisztián Gáspár,Zoltán Hendrik,Christos C Zouboulis,Klaudia Dócs,Péter Szücs,Katalin Dull,Dániel Törőcsik,Anikó Kapitány,Andrea Szegedi

Frontiers in medicine 9:981610 PubMed36148459

2022

Expression of extracellular matrix components in the meibomian gland.

Applications

Unspecified application

Species

Unspecified reactive species

Di Chen,Xiaomin Chen,Hua-Tao Xie,Mark P Hatton,Xiaowei Liu,Yang Liu

iScience 25:104983 PubMed36093061

2022

Heme oxygenase-1 mitigates liver injury and fibrosis via modulation of LNX1/Notch1 pathway in myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giacomo Canesin,Linda Feldbrügge,Guangyan Wei,Lubica Janovicova,Monika Janikova,Eva Csizmadia,Juliana Ariffin,Andreas Hedblom,Zachary T Herbert,Simon C Robson,Peter Celec,Kenneth D Swanson,Imad Nasser,Yury V Popov,Barbara Wegiel

Scientific reports 11:17296 PubMed34453089

2021

Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Yoon Soo Cho,Ji Heon Park,Yu Mi Ro,June-Bum Kim,Cheong Hoon Seo

Biomolecules 11: PubMed34203873

2021

Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Lahiri,Suranjana Mukherjee,Biswajoy Ghosh,Debnath Das,Basudev Lahiri,Shailendra Kumar Varshney,Mousumi Pal,Ranjan Rashmi Paul,Jyotirmoy Chatterjee

International wound journal 18:332-341 PubMed33751815

2021

Epidermal graft encourages wound healing by down-regulation of gap junctional protein and activation of wound bed without graft integration as opposed to split-thickness skin graft.

Applications

Unspecified application

Species

Unspecified reactive species

Muholan Kanapathy,Nadine Hachach-Haram,Nicola Bystrzonowski,David L Becker,Afshin Mosahebi,Toby Richards

Thyroid : official journal of the American Thyroid 30:1066-1078 PubMed32111151

2020

The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina Mancino,Annarita Sibilio,Cristina Luongo,Emery Di Cicco,Caterina Miro,Annunziata Gaetana Cicatiello,Annarita Nappi,Serena Sagliocchi,Raffaele Ambrosio,Maria Angela De Stefano,Daniela Di Girolamo,Tommaso Porcelli,Melania Murolo,Federica Saracino,Giuseppe Perruolo,Pietro Formisano,Mariano Stornaiuolo,Monica Dentice

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 6 antibody [Ks6.KA12]

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (AB18586)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (17)

Product promise