AB53123

Anti-Cytokeratin 7 antibody

Name: Anti-Cytokeratin 7 antibody (ab53123) | Abcam
Brand: Abcam Limited
SKU: AB53123
Price: 545 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal Cytokeratin 7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human KRT7.

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, CK7

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (AB53123)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (AB53123)

ICC/IF image of ab53123 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (AB53123)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (AB53123)

ab53123 at 1/50 dilution staining Cytokeratin 7 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

Unknown

Western blot - Anti-Cytokeratin 7 antibody (AB53123)

All lanes:

Western blot - Anti-Cytokeratin 7 antibody (ab53123) at 1/300 dilution

Lane 1:

HepG2 cell extract

Lane 2:

HepG2 cell extract with immunising peptide

Predicted band size: 51 kDa

Observed band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KRT7. The exact immunogen used to generate this antibody is proprietary information.

P08729

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab53123 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0323255 PubMed40338881

2025

Human and rat ex vivo sweat glands for the observation of acetylcholine induced intracellular calcium signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Franziska Buck,Tobias Kisch,Charli Kruse,Matthias Brandenburger

Lab on a chip 24:1727-1749 PubMed38334486

2024

Endocrine-disrupting compounds and their impact on human placental function: evidence from placenta organ-on-chip studies.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel S Vidal,Lauren S Richardson,Ananth Kumar Kammala,Sungjin Kim,Po Yi Lam,Rahul Cherukuri,Tilu Jain Thomas,Mohammed Bettayeb,Arum Han,Ivan Rusyn,Ramkumar Menon

mBio 12:e0184921 PubMed34399615

2021

Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Siavash Azari,Lauren J Johnson,Amy Webb,Sophia M Kozlowski,Xiaoli Zhang,Kara Rood,Amal Amer,Stephanie Seveau

Nature communications 12:4408 PubMed34344888

2021

Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Yalda Afshar,Jiantong Dong,Pan Zhao,Lei Li,Shan Wang,Ryan Y Zhang,Ceng Zhang,Ophelia Yin,Christina S Han,Brett D Einerson,Tania L Gonzalez,Huirong Zhang,Anqi Zhou,Zhuo Yang,Shih-Jie Chou,Na Sun,Ju Cheng,Henan Zhu,Jing Wang,Tiffany X Zhang,Yi-Te Lee,Jasmine J Wang,Pai-Chi Teng,Peng Yang,Dongping Qi,Meiping Zhao,Myung-Shin Sim,Ruilian Zhe,Jeffrey D Goldstein,John Williams,Xietong Wang,Qingying Zhang,Lawrence D Platt,Chang Zou,Margareta D Pisarska,Hsian-Rong Tseng,Yazhen Zhu

Frontiers in immunology 12:709466 PubMed34367171

2021

Human Placental Trophoblasts Infected by Undergo a Pro-Inflammatory Switch Associated With Poor Pregnancy Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren J Johnson,Siavash Azari,Amy Webb,Xiaoli Zhang,Mikhail A Gavrilin,Joanna M Marshall,Kara Rood,Stephanie Seveau

Cells 8: PubMed30744205

2019

Systematic Generation of Patient-Derived Tumor Models in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Roland Ehrenberg,Jianpeng Gao,Felix Oppel,Stephanie Frank,Na Kang,Sebastian M Dieter,Friederike Herbst,Lino Möhrmann,Taronish D Dubash,Erik R Schulz,Hendrik Strakerjahn,Klara M Giessler,Sarah Weber,Ava Oberlack,Eva-Maria Rief,Oliver Strobel,Frank Bergmann,Felix Lasitschka,Jürgen Weitz,Hanno Glimm,Claudia R Ball

Comparative biochemistry and physiology. Part B, B 225:1-12 PubMed29960082

2018

Monitoring changing cellular characteristics during the development of a fin cell line from Cyprinus carpio.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Rakers,Anne-Kathrin Ondrusch,Martina Gruening,Mikolaj Adamek,Benita Moeckel,Marina Gebert

EMBO molecular medicine 9:918-932 PubMed28526679

2017

Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts.

Applications

ICC/IF, IHC-P

Species

Unspecified reactive species, Unspecified reactive species

Claudia R Ball,Felix Oppel,Karl Roland Ehrenberg,Taronish D Dubash,Sebastian M Dieter,Christopher M Hoffmann,Ulrich Abel,Friederike Herbst,Moritz Koch,Jens Werner,Frank Bergmann,Naveed Ishaque,Manfred Schmidt,Christof von Kalle,Claudia Scholl,Stefan Fröhling,Benedikt Brors,Wilko Weichert,Jürgen Weitz,Hanno Glimm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (AB53123)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (AB53123)

Western blot - Anti-Cytokeratin 7 antibody (AB53123)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise