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Rabbit Polyclonal Cytokeratin 7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Keratin, type II cytoskeletal 7.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (AB53123), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody (AB53123), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (AB53123), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Keratin, type II cytoskeletal 7. The exact immunogen used to generate this antibody is proprietary information. Database link P08729

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/300.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-5.00000 µg/mL

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Alternative names

Recommended products

Rabbit Polyclonal Cytokeratin 7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Keratin, type II cytoskeletal 7.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Keratin, type II cytoskeletal 7. The exact immunogen used to generate this antibody is proprietary information. Database link P08729
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab53123 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (ab53123), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (ab53123)

    ab53123 at 1/50 dilution staining Cytokeratin 7 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

  • Western blot - Anti-Cytokeratin 7 antibody (ab53123), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody (ab53123)

    All lanes: Western blot - Anti-Cytokeratin 7 antibody (ab53123) at 1/300 dilution

    All lanes: HepG2 cell extract

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (ab53123), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (ab53123)

    ICC/IF image of ab53123 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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