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Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for IP, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (AB68460), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (AB68460), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (AB68460), expandable thumbnail
  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (AB68460), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/20

Notes

For unpurified use at 1/50.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/20000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1620Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460), expandable thumbnail

    Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

    ab68460 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
    Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab68460 & T-47D whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab68460 in T-47D whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

    Predicted band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

    Blocking and diluting buffer : 5% NFDM/TBST

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform.[PMID:16265353]

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg/mL

    Lane 2: T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg/mL

    Lane 3: SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg/mL

    Lane 4: Human placenta tissue lysate at 20 mg/mL

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460), expandable thumbnail

    Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

    Western blot: Anti-KRT7 antibody [EP1620Y] (ab68460) staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab68460 was shown to bind specifically to KRT7. A band was observed at 40-55 kDa in wild-type A549 cell lysates with no signal observed at this size in KRT7 knockout cell line. To generate this image, wild-type and KRT7 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/10000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: KRT7 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Observed band size: 40-55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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